Measurements of primary production, esti- 

 mated by rates of carbon fixation by phyto- 

 plankton, were made using standardized C^^ 

 solution (fig. 11, table 5). The solution was 

 made up in the manner described by Steemann 

 Nielsen (1952), except that triple glass-distilled 

 water was used as the carrier instead of 

 artificial sea water. The solution was adjusted 

 to pH 9.0, filtered through a membrane filter 

 and placed in clean 5-ml. ampoules, which then 

 were sealed and autoclaved. 



Filters retaining the incubated phytoplankton 

 were given to the Department of Oceanography, 

 University of Washington, for counting. The 

 activity on the filter was measured with a 

 continuous gas flow geiger counter equipped 

 with a mylar end-window. Dark bottle uptake 

 measurements indicated an accumulated bac- 

 terial population and were not used in calcula- 

 tions of primary productivity. Light bottles 

 were scrubbed daily and had no such accumu- 

 lation. Homes et al (1958: p. 8) previously 

 found that ". . . dark bottle uptake averages 

 10-13% of the uptake in illuminated 

 bottles . . . . " His observation is supported by 

 initial measurements on the July 1961 Cobb 

 cruise. On this basis dark bottle uptake was 

 assumed to be 10 percent of that in light 

 bottles. Since this is offset by the "isotope- 

 effect" correction (Steemann Nielsen, 1952), 

 both terms were omitted in calculation of 

 primary productivity. 



Hydrographic cast data were processed by 

 the Data Collection and Processing Group of 

 Scripps Institution of Oceanography following 

 procedures described by Klein = (table 2). 

 Bathythermograph slides also were processed 

 at Scripps Institution of Oceanography under 

 the supervision of Margaret K. Robinson 

 (table 3). Traces were used to verify tempera- 

 ture -depth configuration obtained from hydro- 

 graphic station data. 



Zooplankton samples were analyzed to iden- 

 tify and determine relative abundance of the 

 organisms present for selection of species to 

 be more closely examined later. Letters which 

 designate abundance of the various taxonomic 



groups (table 8) are relative only to the total 

 in each sample. They have no absolute numeri- 

 cal significance. Groups in parentheses are 

 tentative identifications. 



ACKNOWLEDGMENTS 



Special acknowledgment is made to the Data 

 Collection and Processing Group of Scripps 

 Institution of Oceanography for processing 

 hydrographic cast and BT data and to the 

 Department of Oceanography, University of 

 Washington, for their analysis of salinity and 

 C^^ productivity samples. Also greatly appre- 

 ciated is the loan of hydrographic data by the 

 Department of Oceanography, University of 

 Washington, for verification of horizontal 

 salinity, temperature, and oxygen distribution 

 (figs. 2-7). These data were obtained on M/V 

 Brown Bear cruise 290 from July 6-25, 1961, and 

 will be published by the collecting agency. 



SCIENTIFIC PERSONNEL 



Harold C. Johnson 



Peter Larson 

 Jan B. Lawson 



Robert W. Owen, Jr. 



'Hans T. Klein. A new technique for processing 

 physical oceanographic data. Contributions from the 

 Scripps Institution of Oceanography. New Series, No. 

 000. Undated, typed M.S. 



Fishery methods and 

 equipment specialist. 

 Exploratory Fishing 

 and Gear Research 

 Base, Seattle, Wash.; 

 party chief 



Master, M/V John N. 

 Cobb, Seattle, Wash. 



Senior Marine Techni- 

 cian, Scripps Institu- 

 tion of Oceanography, 

 La JoUa, Calif. 



Fishery Biologist (Re- 

 search), Bureau of 

 Commercial Fish- 

 eries, San Diego, Calif. 



LITERATURE CITED 



ALVERSON, DAYTON L. 



1961. Ocean temperatures and their rela- 

 tion to albacore tuna tThunnus germo^ dis- 

 tribution in waters off the coast of 

 Oregon, Washington and British Colum- 

 bia. Journal of the Fisheries Research 

 Board of Canada, vol. 18, no. 6, p. 1145- 

 1152. 



16 



