Notea on Cruises 1 through 3 



It will be noticed that samples for 

 organic determinations were taken at three 

 levels at alternate stations, and that 

 total phosphorus was not included. These 

 early collections were made to determine 

 the feasibility of freezing the samples at 

 sea with subsequent analyses being made 

 ashore after a cruise was completed. IXir- 

 ing these early cruises we were also short 

 of personnel and it would have been impos- 

 sible to analyze full complements of 

 samples as in the later cruises. 



Note on Cruise 1 



The carbohydrate values for Cruise 1 

 at the deeper levels are almost all indi- 

 cated as "green". This means that the re- 

 acted samples were green to the eye instead 



of the normal reddish-purple. However, the 

 filters used in the photometer prevented 

 the measurement of the light transmitted in 

 the green portion of the spectrum, hence 

 some green values are given sis zero carbo- 

 hydrates. The fact that the green response 

 all but disappeared in the later analyses 

 would indicate that an unknown technical 

 difficulty caused the reaction to yield 

 green. The values are included for the sake 

 of completeness, and because, in spite of 

 the discoloration, I feel that they are 

 still representative of some measurable com- 

 ponent present in the sea water. The exist- 

 ence of values of similar magnitudes in the 

 deeper levels at stations occupied during 

 the later cruises supports this contention. 

 There is a possibility that high nitrate- 

 nitrites will cause a green reaction to 

 N-ethyl carbazole reagent in the absence of 

 carbohydrates. 



Figure 15. — Locations of stations occupied on Cruise 1-lA. 



17 



