Eeckhout and Gillis (1953). 



Arabinose equivalents of carbohydrates 

 were determined by the N-ethyl-carbazole 

 method of Erdraan as modified by Zein-Eldin 

 and May (1958). 



Tyrosine equivalents of protein con- 

 tent were determined by a method developed 

 by Peter J. Wangersky as modified by Zoula 

 P. Zein-Eldin and B. Z. May in which the 

 amino-acid content equivalent to tyrosine 

 is obtained by hydrolysis in 0.375 N sodium 

 hydroxide solution. 



The determination is carried out as 

 follows: 



1. Thaw frozen samples. 



2. Pipette duplicate 20-ml. samples 

 with a volumetric pipette into 50-ml. glass 

 reagent bottles with ground glass stoppers. 



3. Add 20 ml. of 0.75 N sodium 

 hydroxide from an automatic burette. 



hours. 



4. Autoclave at 80 pounds for 2-3 



Centrifuge for 20 minutes. 



6. Read on the Beckman spectropho- 

 tometer, using the optical density scale. 

 Set wave length at 330 mu and use the ultra- 

 violet light source. Use the 5 cm. silica 

 cells and read against distilled water. 



7. Treat blanks and samples of tyro- 

 since (1, 3, 5, mg. per liter) similarly. 

 Blanks are made up in protein-free artifi- 

 cial sea water. Standards are made up in a 

 low protein natural sea water, if available; 

 otherwise, artificial sea water is used. 



ACKNOWLEDGMENT 



The authors are indebted to numerous 

 co-workers for their advice and assistance 

 in assembling the data contained in this 

 report. K. T. Marvin, Z. Zein-Eldin, 

 B. Z. May and L. Lansford made the chemical 

 analyses and determinations. M. W. Jambor 

 assisted in enumeration of G. breve and in 

 tabulating data. 



Albert W. Collier developed the sam- 

 pling methods and William B. Wilson devel- 

 oped the enumeration techniques. Joe 0. 



Bell was in charge of the field work until 

 March 1957. 



The Florida State Board of Conserva- 

 tion, especially Ernest Mitts, Director, 

 Robert M. Ingle and Dr. Robert F. Hutton 

 cooperated in numerous instances by supply- 

 ing patrol vessels and crews and other 

 facilities. 



LITERATURE CITED 



GALTSOFF, PAUL S. 



1948. Red tide. Progress report on the 

 investigations of the cause of 

 the mortality of fish along the 

 west coast of Florida conducted 

 by the Fish and Wildlife Service 

 and cooperating organizations. 

 U. S. Fish and Wildlife Service, 

 Special Scientific Report No. 46, 

 44 pp. 



GRAHAM, HERBERT W. , JOHN M. AMIS0N, 

 AND KENNETH T. MARVIN 

 1954. Phosphorus content of waters 



along the west coast of Florida. 

 U. S. Fish and Wildlife Service, 

 Special Scientific Report :Fisher- 

 ies No. 122, 43 pp. 



GUNTER, GORDON, ROBERT H. WILLIAMS, 



CHARLES C. DAVIS, AND F. G. WALTON 

 SMITH 

 1948. Catastrophic mass mortality of 

 marine animals and coincident 

 phytoplankton bloom on the west 

 coast of Florida, November 1946 

 to August 1947. Ecol. Monogr. 

 18(3) :309- 324. 



HARVEY, H. W. 



1948. Estimation of phosphorus and 

 total phosphorus in sea water. 

 J. Mar. Biol. Assoc. U. K. (27): 

 337-359. 



HOSTE, J. , J. EECKHOUT, AND J. GILLIS 

 1953. Spectographic determination of 



copper with curroine. Analytica 

 Chemica Acta. (9):263-274. 



HUTTON, ROBERT F. 



1956. An annotated bibliography of red 

 tides occurring in the marine 

 waters of Florida. Quart. Jour. 

 Fla. Acad. Sci. Nos. 2-3, (19): 

 124-146. 



