CONTENTS 



Page 



Introduction 1 



Material 2 



One -dimensional chromatograms 2 



Procedure 2 



Results 3 



Two-dimensional chromatograms 4 



Procedure 4 



Results 4 



Discussion and conclusions 6 



Summary 8 



Bibliography 9 



ILLUSTRATIONS 



FIGURE Page 

 Frontispiece: Chromatograms being developed in chromatocab 



1. One-dimensional chromatograms of adult tuna samples developed in two different 



solvents 3 



2. One-dimensional chromatograms of adult and larval samples of two species of tuna 



developed in n-butanol:methylethylketone:ammonia:water 4 



3. Two-dimensional chromatogram of adult little tunny 4 



4. Two-dimensional chromatogram of adult albacore 5 



5. Two-dimensional chromatogram of adult bigeye 5 



6. Two-dimensional chromatogrann of adult yellowfin 6 



7. Two-dimensional chromatogram of adult skipjack 6 



8. Two-dimensional chromatogram of adult bigeye run in reverse order of solvents ... 7 



9. Two-dimensional chromatogram of adult yellowfin run in reverse order of solvents. . 7 



10. Two-dimensional chromatogram of larval yellowfin 8 



11. Two-dimensional chromatogram of larval skipjack 8 



