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FIRST DIRECTION «-euTANOL: METHYLETHYLKETONE : AMMONIA : WATER - 24 HOURS 



ever, inasmuch as this would involve a specific 

 action between solvent and acid, it is logical to 

 assume that identical acids were lost by all of 

 the species. If so, this discrepancy should not 

 affect the final results. 



Both good and poor results were ob- 

 tained from the samples of larval tunas. In the 

 case of yellowfin (fig. 10), no trace was obtain- 

 ed with samples of less than 5 larvae and even 

 with this number, the sample size seemed to be 

 too small. Perhaps the use of more larvae 

 would have given better results, but the supply 

 of larval yellowfin was depleted at this stage of 

 the investigation and further tests were not pos- 

 sible. 



In the case of skipjack (fig. 11), how- 

 ever, better results were obtained. The figure 

 shows the chromatogram of a sample of 7 larvae , 

 their sizes ranging from 4.5 to 8.0 nnm. Al- 

 though not all of the amino acids shown by the 

 adults were obtained from the larvae, a suffi- 

 cient number was developed to show some simi- 

 larity in the overall patterns of the dominant 

 annino acids. 



Figure 4. --Two-dinnensional chromatogram of 

 adult albacore. 



A second testing of the bigeye and yellow- 

 fin samples produced better separation of the 

 spots (figs. 8 and 9). In this instance, the or- 

 der of solvents was inadvertantly reversed, but 

 it is not believed that this had any adverse ef- 

 fect on the chromatograms. Both species show- 

 ed slightly different patterns than those run in the 

 original order of solvents. However, the dif- 

 ferences, if any, between the two species were 

 insignificant. 



Some discrepancy is noted in the number 

 of spots obtained with identical solvents between 

 the one- and two-dimensional chromatograms, 

 i.e., the two-dimensional chromatograms con- 

 tained less spots in each of the solvents than 

 the one-dimensional chromatograms run in 

 identical solvents. This discrepancy could per- 

 haps be due to difference in the time of runs 

 (1 nnonth apart) between the two tests. There is 

 also the possibility that some of the amino 

 acids separated in one solvent may have been 

 lost in the other solvent in the two-dimensional 

 tests. Because the annino acids were not iden- 

 tified, it is not possible to say whether the 

 acids lost in each of the two dimensions were 

 of the sanne type for all of the samples. How- 







o 



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FIRST DIRECTION rt-BUTANOL METHYLETHYLKETONE AMMONIA WATER- 24 HOURS 



Figure 5. --Two-dimensional chromatogram of 

 adult bigeye. 



