CONTENTS 



Page 



Records of bacterial diseases of fish before 1894 2 



Description of causative organism 4. 



Classification 4, 



Morphology and staining. 4 



Viability. . . , . , 5 



Temperature, light and drying 5 



In water. . . . , 5 



In sewage . . o . . . . 7 



Undiluted fresh sewage . 7 



Undiluted sewage sterilized ty Berkefeld filtration. ... 8 



Autoclaved sewage 9 



Ether-treated sewage . 9 



Saprophytic existence and survival of B. salmonicida 



in dead fish -. 10 



Cultural characters 10 



Relation to free oxygen 10 



Agar. 10 



Broth 11 



Potato 12 



Pigment production , 12 



Salt stability. 13 



Biochemical reactions 13 



Gelatin 13 



Serum media 1^ 



Milk. . Li 



Carbohydrate reactions. I4, 



Other biochemical reactions I4. 



Agglutination of B. salmonicida with fish antisera I9 



B. salmonicida complement fixation .. I9 



Variability of B. salmonicida I9 



Biological characters . 19 



Dissociation — formation of S, R, and G colonies 21 



Dissociation in phenol broth series 21 



Summary of B. . salmonicida dissociation in phenol broth 



series, . ...... 22 



Dissociation in lithium chloride broth series 23 



Appearance of G-type cultures. ...... 23 



Recovery of tjT^ical B, saljionicida from G-variants .... 24 



Serological relationships of S, R, and G phases 25 



l^ing additional strains o . . , 25 



Antigenic relationships of the G variants to P. and 3 var- 

 iants 26 



Bacteriophage in relation to causative organism 26 



In water » ......... . 26 



Technique in isolation and demonstration 27 



Occurrence 27 



