Table 2 indicates that in general B. salmonicida grows rapidly 

 on gelatin with liquefaction occurring in most cases, from 21^ hours to 

 a few day So Pigment is relatively common, being recorded after the 

 third day to 20 dayso Gas is reported in only two cases. The shape 

 of the train of liquefaction is variably reported as craterifonn, 

 infundibular form;, stratiform, and sacculate. 



Serum media 



Table 3 shows the results of various authorities on the reaction 

 of B. salmonicida on solidified serum with respect to growth, 

 liquefaction and pigment production. 



Table 3 shows that B. salmonicida grows rapidly on solidified 

 serum with liquefaction occurring from the second to fourteenth day. 

 Pigment production also occurs, variably from the third to fourteenth 



Milk 



Table 4 shows that in general B. salmonicida grows in milk, 

 sometimes abundantly; brings about peptonization of milk ivith acid 

 productionj does not coagulate milk. Pigment production occurs in about 

 20 days as in the case of Duff and Steivart (1933), but it is not a 

 prominent feature as five authorities make no mention of it. Stevens 

 and Keil (1931) record no change whatsoever in milk. 



Carbohydrate reactions 



Table 5 siimraarizes the car-bohydrate reactions of B. salmonicida . 



Table 5 shows tnat in general glucose and mannite are fermented 

 and that sucrose, lactose, and dulcite are not fermented. In addition 

 Mettam (191A), B-iff and Stewart (1933) found that galactose, levulose, 

 maltose, and salicin were also fermented but that raffinose was not. 



Other biochemical reactions 



According to Duff and Stewart (1933), starch is utilized in 

 Eckford's broth. An initial pH of 7.0 is reduced to 6.0 in 2 days and 

 to 5. A in 9 days. It is also utilized in starch agar. 



Nitrates are reduced to nitrites in 5 to 7 daj'-s (Marsh 1903, Duff 

 and Stewart 1933)0 According to Marsh (1903) 7-day cultures contain 

 both nitrites and ammonia. He also found that AO-day cultures contain 

 no nitrite but give a strong test for ammonia. 



14 



