Table 8. — Agglutination of R and S phases of strains of Groups I and II 

 by CL6 monovalents "n" servani / 



1/ Agglutination was at 37° C. for 4 hours. Results were read after 

 overnight ice-box storage. 



Antigenic Relationship of the G Variant to R and S Variants 



Duff (1939) felt that the G phase cell might be composed in part 

 of an individual G antigen or antigen complex^ and in part of an antigen 

 or antigens common to itself and to R and S cells of the same strain. 

 He made no claim that all strains of B. salmonicida would necessarily 

 fall into one of two serological groups in respect to the location of 

 the n antigen in the R or S phase of the strain under consideration. 

 Nevertheless^ it is significant that 19 strains selected at random did so. 



He also found that pathogenicity was not correlated with the 

 antigenic picture elicited by the absorption methods used in his 

 experiments. 



BACTERIOPHAGE. IN RELATION TO CAUSATIVE ORGANISM 



In Water 



Christison and associates, who carried out a thorough study on 

 B. salmonicida bacteriophage in I9385 collected water in a sterile 

 stoppered bottle and added 200 cc. to 100 cc. triple-strength broth in 

 each of five large flasks.^ carefully avoiding any contamination. One 

 liter of water was used therefore in each case. Twelve strains of B. 

 salmonicida isolated from cases of furunculosis in salmon and trout were 

 chosen at random from a large selection of stock strains. Twenty-four 

 hours' agar slope cultures of these were emulsified in broth and the 

 emulsions were added to the broth-water mixt.ures in the flasks, three 

 different strains being added to each flask. Since only twelve strains 

 were employed^ the fifth flask was inoculated with three of the strains 

 already used„ but in a different combination. In a number of cases the 

 fifth flask was omitted, 1000 cc. of water being distributed in equal 

 quantities among four flasks^ in each of which 125 cc, of triple- strength 

 broth had been placed. 



26 



