goldfish were inoculated subcutaxieously -with a pathogenic- vibrio^ 

 progressive congestion of vessels around the site of inoculation was 

 observed and films of subcutaneous tissue showed that migration of 

 leucocytes had taken place and that definite phagocytosis was occurring. 



Table 9,— Features of retaining-pond disease, British Columbia- 



1/ 



Pathological feature (naked-eye observation). 



Muscle-- large (0,5 cm. to 10 cm.) closed 



lesion, containing fluid or semifluid 

 reddish ''pus'ij subcutaneous or deepo 



Muscle— large lesions open to the exterior 

 but not located at the base of the 

 pectoral or pel-vic fins. 



Muscle— small heramorrhagic dots -^r streaks ir 

 Kidney— liquefactive necrosis of^ 

 Spleen— liquef active necrosis of c 

 Liver—hemorrhage or lesion of ^ 

 Intestine-— congestion of any portion of. 

 Intestine— marked congestion of posterior 



part with or -without congestion 



of other portions ^ 

 General visceral congestion or peritonitis^ 

 Free body fluid in body cavity, 

 Vent'-- exudate of blood and mucus from. 

 Pelvic fins— subcutaneous hemorrhage at base 



of? 

 Pelvic fins— open lesions at base of^, 



Frequency of 

 occurrence c 



27 Number of fish show- 

 ing one or more 

 types of muscle les- 

 ions- — 66 o 



10 



32 Total lesions at 

 8 base of pelvics— 

 4.0 



Pectoral fins— subcutaneous hemorrhage at base 



Pectoral fins— open lesions at base of. 



Anal fin—subcutaneous hemorrhage at base of_. 



19 Total lesions at 

 1.5 base of pectorals 



--3A. 

 22 



1/ 



After Duff and Stewart. (1933). 



No active toxin or filterable agent has been demonstrated in 

 fiirunculosis (Furunculosis Committee 1935). Filtrates of a spleen of 

 an infected fish were injected into two fish but no signs of disease 

 developed even after 1 month. Two control fish inoculated with unfiltered 

 emulsion each died in 5 days with B, salgioni cida being recovered from 



34 



