that might be carried over the surface (Snieszko and Friddle 1948). On 

 the other hand, the action of the disinfectant should not be affected by 

 the presence of proteins (Snieszko and Friddle 1948). 



Mary chemicals have been tried as external disinfectants of trout 

 eggs and Gee and Sarles (1942) listed a number of them in the order in 

 ■which they proved useful. The solution percentages given are for use in 

 10-minute treatments of eggs. 



Disinfectant Percent 



solution 

 Reconmendeds 



1. Sulfo-merthiolate (Lilly) 0.015 



2. Acriflavine 0.185 



Safe: 



3. Mercuric chloride 0.0025 



4. Mercurochrome 0.68 



5. Chlorozene (Abbott) 0.009 



6. Mercarbolide (Upjohn) 0.0052 



7. Calcium hypochlorite (B-K powder) 0.026 



Usable bxit dangerous; 



8. Iodine 0.0056 



9. Azochloramid (Wallace & Tiernan) 0.001 



10. Gentian Violet 0.15 



11. Sodium hypochlorite (KLenzade) 0,062 



Extremely dangerous: 



12. Formaldelgrde , 0.53 



13. Metaphen 0.0099 



Not usable: 



The list shown above (Gee and Sarles 1942) indicates clearly that 

 the best trout egg disinfectants are sulfo-merthiolate and acriflavine. 

 Therefore,, some space -will be devoted to their general application, but 

 the author does not feel it necessary to deal in detail mth the techniques 



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