Three daphnia ponds were each stocked with 50 bluegills 

 ( Le ponds macrochirus ) averaging 1.0 inches in length on August 5, 

 19U1 » Two of these ponds were sprayed on August 6, with oil 

 spray (formula 6) at the rate of 0,25 pound per acre (0,0^ p.p.m«)j 

 the tnird pond remained untreated. The average temperature during 

 the experimental period, August 6 to 10 was 72,9° F. The heaviest 

 mortality (about 70 percent) occurred three days after treatment. 

 \ih.en the ponds were drained on August 10, 80 percent of the fish 

 were dead in one sprayed pond and 90 percent in the other, but no 

 fish died in the control pond. 



Several dirt-bottomed ponds contained adults and recently 

 hatched fry of the golden shiner ( Notemigonus crysoleucas ) . "Hiese 

 young fish ranged from 0.3 to 1.1 inches in length. Since fry of 

 this size cannot be handled readily, a count was made of the schools 

 of young fry about the borders of four ponds to note their presence 

 and relative abundance before spraying. 



On June 20, 1947, pond 10 was sprayed with DDT in oil (formula 6) 

 at 0.5 pound per acre (0.065 p.p.m.). Affected fry irere found about 

 the entire shore line 24. hours later. Their nervous reactions often 

 left them stranded more than an inch from the water's edge. As far 

 as could be determined, all fry were killed, but the adults survived. 



A second pond (13) containing golden-shiner fry was sprayed at 

 the same time at the rate of 0.25 pound per acre (0.025 p.p.m.). No 

 young fry could be found two days later. 



A third pond (11) was sprayed with oil spray (fonmila 6) at 0.125 

 pound per acre (0.014 p.p.m.) on June 30, 1947. Fry were observed 

 for nine days after treatment, and were apparently unaffected by the 

 dosage. During this period the golden-shiner fry in control pond 7 

 remained in evidence. The average ten^rature for these ponds dur- 

 ing this period was 72.0° F. 



Suspensions and Oil Formulations 



Nine dirt-bottomed ponds were stocked with 600 fish — 200 each 

 of fingerling bluegills, brown bullheads (Ameiurus nebulosus ) . and 

 black crappies (Pomoxis nigro-maculatus ) hatched during the 1946 

 season. The young bluegills had an average weight of 0,56 pound per 

 thousand (1.0 inch in length), black crappies 0.34 gram each (1.2 

 inches), and brpwn bullheads 0.17 gram each (length not recorded). 

 Six ponds were sprayed July 11 and all were drained on July 19 • 



