Partial reconstltution of the dry meal was accom- 

 plished by adding 30 percent water to the diet 

 during mixing. No water was added to the other 

 three diets, (Diets 9, 10 and 11). 



Salmon offal meal at the 5 percent level (Diet 9) 

 contributed significantly more growth stimulus during 

 the first 12-week period than did a like amount of dis- 

 tillers solubles (Diet 10). The control for this group 

 (Diet 8) which contained no meal produced gains com- 

 parable to Diet 9 . The addition of 10 percent of salmon 

 offal meal to these diets during the second 12-week 

 period did not produce any significant differences in 

 final weights among the three diets . 



Distillers solubles at the 5 percent level proved 

 to be an excellent vitamin supplement. This product, 

 while not contributing measurably to growth production, 

 prevented an anemic tendency in the fish of Diet 10. 

 The fish of both Diets 8 and 9 which did not contain dis- 

 tillers solubles had low amounts of hemoglobin . 



The inclusion of comparatively high levels of meals, 

 again, proved to be detrimental during the cold-water 

 period. The addition of 15 percent of distillers solubles 

 (Diet 11) caused a reduction in growth and an increase in 

 mortality . The diet containing 50 percent of dry meal 

 (Diet 12) made the poorest growth of any in the experi- 

 ment . Mortalities were significantly higher than any 

 other with the exception of Diet 11, however no de- 

 ficiency symptoms were evident at the end of the 24- 

 week period. 



It may be concluded from the results of this experi- 

 ment that heat treated salmon viscera was inferior to 

 raw viscera in growth potential. Arrow -toothed halibut 

 proved superior to squawfish in diets containing 50 per- 

 cent fish products and may be considered an adequate 

 substitute for salmon viscera in blueback salmon diets . 

 Dry meals in excess of 5 percent did not prove satis- 

 factory at water temperatures below 50° F. Five per- 

 cent distillers solubles added to a diet was effective 

 in preventing an anemic tendency which was found in 

 similar diets not containing this proportion of distillers 

 solubles . 



Chinook Salmon 



The emphasis in the 1954 chinook feeding trials was 

 placed on 50 percent meal diets using previously tested 

 rations containing 10 percent meal as controls . The 

 Cortland No. 6 diet was used as the base and variations 

 were made in the meal mix in one phase and in the meat 

 components in the other phase. These diets were partial- 

 ly reconstituted by the addition of 30 percent water . 



A coccidia-like intestinal infection caused an increase 

 in the mortality during the first 12 weeks of the trials but 

 was successfully controlled by sulfa riierapy. Higgler 

 than normal mortalities, however, resulted and tended 

 to obscure the results of the experiment. 



The standard meat -viscera mixture with 5 per- 

 cent each of seal meal and distillers solubles (Table 3, 

 Diet 1-C) was used as one of the control diets . This 

 diet again produced excellent growth with no deficiency 

 symptoms . The second control (Diet 2-C) was a pre- 

 viously tested combination of 22.5 percent each of hog 

 liver and arrow -toothed halibut, 45 percent of salmon 

 viscera and 10 percent of the seal meal - distillers 

 solubles mix. Similar results were obtained with this 

 diet as with Diet 1-C. 



The high meal diets may be divided into two groups. 

 In the first group (Diets 3-C through 6-C) the meal 

 components remained the same and the meats were 

 varied. The meal mixture was essentially the Cort- 

 land No. 6a mix: equal parts of wheat middlings, 

 cottonseed meal, distillers solubles and salmon offal 

 meal. The Cortland No. 6 diet with 15 percent beef 

 liver and 35 percent hog spleen (Diet 3-C) acted as the 

 control for this group. Salmon viscera was the only 

 meat component in Diet 4-C. Equal parts of hog liver 

 and beef lung (Diet 5-C) and hog liver and salmon vis- 

 cera (Diet 6-C) completed this group of diets . 



None of the diets in the meat-variable group pro- 

 duced good growth but of the four, the Cortland diet 

 (Diet 3-C) and the hog liver - salmon viscera variable 

 (Diet 6-C) showed the best gains at the end of 12 weeks. 

 The other two diets produced very poor growth with 

 Diet 4-C actually losing weight . This loss of weight 

 was due to a mortality in excess of 50 percent at the 

 end of 12 weeks. During the sixth week, moribund fish 

 from this diet (Diet 4-C) were examined and the pres- 

 ence of clubbed gills indicated a pantothenic acid 

 deficiency. 



The results at the end of the 24-week period were 

 very similar to those at the end of 12 weeks . One diet 

 (Diet 3-C) was discontinued during the sixteenth week 

 when silt-clogged water inflow pipes caused a heavy 

 mortality. The other three diets, however, main- 

 tained the same positions in amount of growth with the 

 final weight of fish in Diet 6-C exceeding significantly 

 those of Diets 4-C and 5-C. EXiring the gross examina- 

 tion of the fish at the end of the experiment, both Diets 

 4-C and 6-C contained some fish with clubbed and fused 

 gills - evidence of a previous pantothenic acid deficiency. 



The hog liver - salmon viscera components re- 

 mained the same in the second group of diets (Diets 6-C 

 through 9-C) and the meal mix was varied. The Cort- 

 land meal mixture (Diet 6-C) acted as the control for 

 this group. Seal meal was substituted for salmon offal 

 meal in Diet 7-C. In the last two diets (Diets 8-C and 

 9-C) both the salmon offal meal and seal meal were 

 deleted and the distillers solubles increased. 



In the meal variable group of diets, seal meal (Diet 

 7-C) provided as much or more growth response than 

 did salmon offal meal (Diet 6-C) . The increased amounts 

 of distillers solubles in Diets 8-C and 9-C however 



