Manchester Memoirs, Vol. Ivii. (1913), No. 8- 3 



Method. 



Killing and fixing reagents used were weak Flemming's 

 solution, and Chromo-Acetic acid (SchafFner's formula), 

 The material was taken through ten per cent, glycerine 

 to absolute alcohol, cleared in chloroform, and embedded 

 in wax with a melting point of 54°C. Microtome sections 

 were cut varying from 2-8 fx in thickness. The stain 

 found to give best results was Heidenhain's iron h^tma- 

 toxylin with bisniark brown, light green or erythrosin as a 

 counterstain. Man}^ observations were made upon thickly 

 cut hand sections and macerations of fresh and preserved 

 material. 



Mycelium. 



W'oronin describes the mycelium of Lcptosplupria 

 Leviancce as being " strongly developed in the hollow 

 central portion of the thallus, but extending into the 

 parenchymatous tissue where fructification occurs. For 

 the most part the tissue is only affected between the 

 cells. The hyphas are fairly fine, delicate, hyaline, and 

 possess numerous cross walls. Their branchings are very 

 irregular, and at points of contact fusion not infrequently 

 occurs." 



This desci'iption is in accord with my observations, 

 but the mycelium perhaps merits a fuller treatment. 



The hypha; within the cylinder of the host run in a 

 generally longitudinal direction and are more or less 

 confined to the thick layer of mucilage lining the lumen. 

 Frequently, however, strands are applied about the axial 

 filament. These hyphse shew two forms. The one 

 consists of fine, much-branched threads, which b\' fusion 

 at points of contact and by bridge connections, form an 

 anastomosing cylindrical network ; the other of fewer, 

 stouter threads, little branched, and usually pursuing a 



