10 J5RIEKLEV, Life-History of Lcptosphceria LevianecE. 



To this concise and accurate description I can add 

 little. 



The perithecia occupy the radial thickness of the 

 host tissue, and, although not infrequently contained 

 within a single medullary cell, more usually occupy two 

 or more. (^Text-fig. 4.) Toward the attenuated ends of 

 the Lemanea shoot the cells are smaller, and here the 

 perithecia, apparently of constantly slightly greater size 

 (■o6-'07 mm.), occupy part or the whole of the lumen. 

 Two or three perithecia often develop in contiguity, and 

 in such cases the perithecial wall frequently remains 

 undeveloped at the point of contact. The result is a large 

 compound perithecium, opening by the several ostioles. 

 Very rarely instances may be found in which the peri- 

 thecia shew reversed orientation, that is, the bulbous base 

 is toward the periphery of the shoot. In such cases the 

 ostiole is undeveloped, and the asci which are directed to 

 the lumen do not dehisce, the spores germinating /;/ situ. 

 In the so-called hymenial layer of the fruiting body asco- 

 genous hyph^e, usually containing paired nuclei, may be: 

 seen. {PL /., Fig. 15.) These continually develop, so that 

 various stages in ascus growth from binucleate dilated 

 tips to mature asci are to be found within the same peri- 

 thecium. The younger asci are situated principally at 

 the periphery, and development proceeds in a centripetal 

 direction. The most careful examination of the nuclei 

 failed to reveal the nuclear membrane, and all that could 

 be seen was the prominent nucleolus, usually surrounded 

 by a clear area of protoplasm. Uninucleate asci of 

 different sizes and asci containing spores in various stages 

 of development are numerous, indicating that these stages 

 last for some considerable period of time. 



Woronin describes briefly "the division of the nucleus, 

 which is usually in the middle of the sac, into eight, the 



