Manchester Memoirs, Vol. Ivii. (191 3), No. 8 15 



infecting hy'pha figured by Woronin is penetrating a 

 cortical cell ; whilst all the cases I examined shewed that 

 the infection occurred in an intercellular manner, and 

 a slight dilation or appressorium is present where the 

 germ tube comes into contact with the host. {Text- 

 fig- 3, I) 



Attempts were made by placing cultures under 

 simulated natural conditions to prolong their life, but 

 three weeks was the maximum period for which they 

 could be retained. 



A large series of hanging drop cultures were made. 



In distilled, sterilised and tap water the spores gave a 

 small amount of long, attenuated hyaline mycelium, with 

 little protoplasmic contents and few walls. The spores 

 became emptied of matter and the walls appeared to 

 become thinner. Hyphal dilations were present in the 

 mycelium, and death ensued after seven to ten days. 



In various nutrient media the cultures were more 

 successful. Dehisced spores, mature asci with spores 

 m situ, asci with two and one-celled spores, and even asci 

 with undifferentiated contents readil)' germinated. {Text- 

 fig- 3. 2.) 



l^eerwort cultures gave a curious initial growth of 

 stout, irregular, much-branched hyphcc, with short rounded 

 cells and tapering hyphal tips. The later growth was 

 more regular. {Text-fig. 3, 3.) 



A similar type of growth was obtained upon bean and 

 prune decoctions. 



Attempts to obtain decoctions oi Levianea shoot were 

 unsuccessful. 



Peptone cultures developed a regular freely-branched 

 mycelium of vigorous growth, with frequent hyphal 

 dilations. {Text-fig: i, /[.) 



