1 6 Bkierlev, Life-History of LeptospJia'via Lemanece. 



In all cases a dense weft of mjcelium, thinning out 

 to the edges, was ultimately obtained. 



Entire perithecia which were placed in hanging drops 

 of culture medium, gave rise to secondary hyphc-e, but no 

 spores were ejected ; and on subsequently opening the 

 fruiting bodies the contents of the asci were found to have 

 germinated /'// situ. 



Cultures in various states of development and vigour 

 were placed under different conditions of temperature, 

 light, and food supph-, but sexual reproduction could not 

 be induced. 



A large series of experiments were carried out, using 

 a refinement of Woronin's method, and that of hanging 

 drops, in attempting to infect aquatic plants other than 

 Levianea. Hosts supplied were Oedogoniiim, Vaucheria, 

 Spirogyra, Cladophora, Ciiara, Nitel/a, Riccia, Fojitinalis 

 E/odea, Myriophylluvi, and Ceratophylhun. The attempts 

 were unsuccessful. 



Distribution. 



From the fact that infected Lcmanca has been collected 

 during nine months of the year, and in each case all 

 stages of perithecial development and mature spores have 

 been present, it would appear that L. LevianecB does not 

 shew seasonal periodicit)' in cither vegetative or repro- 

 ductive development. 



The fungus has been found upon Lcmatiea toriilosa 

 and L. catcuata, d.s well as L. fluviatilis, d^nd appears to 

 be not uncommon wherever the alga grows profusely. 



I'ATIIOLOGICAL EFFKCTS. 



Externally the Lcuianca shows little appearance of 

 disease, the rich deep green colour of the thallus giving 

 place only to a slightly olivaceous hue. Usually the more 



