Immediately prior to drawing of the sample, 

 each bottle was rinsed three or more times 

 with sea water collected at the sample depth. 



The data presented in these pages have been 

 corrected for the isotope effect, but not for 

 respiration or dark-bottle uptake. Replicate 

 values are given when two or more samples 

 were obtained from a given depth and dark- 

 bottle uptake as well. The total CO2 concen- 

 tration of sea water was assumed to equal 

 90 mg./l. and all of the productivity calcula- 

 tions have been made using this value. 



The in situ vertical measurements of produc- 

 tivity were carried out in the following manner. 

 A water sample was collected at each desired 

 depth with the plastic Van Dorn-type sampler 

 shortly before daylight. The samples were 

 transferred to clean, well-aged, 125-ml. Pyrex 

 bottles and the C 14 solution injected with a 

 plastic hypodermic syringe and stainless steel 

 needle. The samples were resuspended at or 

 slightly before dawn, at approximately the 

 depth at which they were collected, on a 

 weighted rope supported by a free-floating 

 glass buoy (14 in. in diameter) enclosed in a 

 cord netting, or by a series of plastic floats 

 which were attached to a bamboo pole bearing 

 a flag and radar reflector at its top. The 

 surface sample was attached to the side of 

 the glass buoy, or plastic floats, just under 

 the sea surface. The samples were collected 

 at noon, local time, and were promptly filtered 

 and dried for counting. The data have been 

 tabulated under the heading "In Situ". 



The samples incubated on shipboard were 

 inoculated with C* 4 in the same manner as 

 the in situ material. The incubator itself was 

 similar to that employed by Steemann Nielsen 

 (1952). Temperature control was achieved by 

 circulating subsurface sea water through the 

 water bath at a rate of 4 to 6 1. per minute. The 

 temperature in the bath fluctuated somewhat 

 but never exceeded the sea-surface tempera- 

 ture by more than 2.3° C, and usually by less 

 than 1° C. Temperatures less than that of the 

 sea surface were not observed in the incubator. 

 The samples were illuminated by a bank of 10 

 daylight-type fluorescent lamps. The intensity 

 of the lamps was adjusted to 1,000 foot-candles 

 by using a continuously adjustable 



autotransformer (Variac) in the lamp circuitry. 

 The data have been tabulated under the heading 

 "Lab. Incubator". 



Surface productivity ("in situ") was measured 

 in one of two ways. On SCOT (TO-58-1) the 

 samples were inoculated with C 14 and trailed 

 astern of the vessel just under the sea surface 

 from local noon until sunset or, less frequently, 

 from sunrise until local noon; the data have 

 been tabulated under the heading "Trailing 

 Bottle" in appendix 1. On TO-58-2, TO-59-1, 

 and TO-59-2 surface water was placed in 

 125-ml. Pyrex bottles, inoculated with C 14 and 

 placed in a deck incubator. The deck incubator 

 was located in a position to yield minimum 

 shadow disturbance. Temperature regulation 

 was achieved by circulating surface sea water 

 through the unit at about 3 to 5 gallons per 

 minute. 



On TO-59-1 (stations 27 to 51) the photo- 

 synthesis of samples from additional depths 

 were measured in the deck incubator. Neutral 

 filters were placed above these samples. The 

 percent transmission in the visible range is 

 given adjacent to the photosynthesis values in 

 the tables. 



Data on the above-mentioned "deck incu- 

 bator" samples, surface and subsurface, have 

 been tabulated under this heading. 



Phytoplankton net sample 



A Hensen-type net with a mesh size of 32 /U., 

 and mouth diameter of 17 cm., was hauled 

 vertically from 50 m. to the surface. 



Zooplankton standing crop 



The measurements reported are all dis- 

 placement volumes of formalin-preserved ma- 

 terial per 1,000 m.3 of water strained, the 

 latter being estimated by flowmeter mounted 

 in the mouth of the net. They refer to oblique 

 hauls by non-closing net with a mouth diameter 

 of 1 m. (N-C N, O), horizontal hauls by the 

 same net (N-C N, H), and horizontal hauls by 

 a modified Clarke-Bumpus net [C N (C-B), H]. 



The nonclosing meter net is the standard net 

 of the California Cooperative Oceanic Fisheries 



