Tliese results -Indicate that the ar.ounr, of noisnri in tho ti.spuGs of the fish tersSr^d 

 -.as very slif^hto It is regrettable that -•'.ere V3.rulent species v;erc5 not selecstedo 



■'"detracts ^lere prepared for comnarison as follovjs usin?; salted dried specimens o 

 The sa'ne ^est material was used in all cases. 



(1) 200 cc of water T7nR added to 10 p;r of pulverize 1 dried salted tissues and 

 the mixture was heated at 100° C for about two h6urs<, After filtering it was con- 

 centrated to 15 cc (0.66 gr of dried tissue to 1 cc of fluid 3n,1'?cted). The re- 

 sults of this experinnent (iTonarntion No. 1) indicates thnt 0.33 tr of flesh is 



a lethrl dose (calculated for a nouse of 15 '^r bodyivei'^ht) . 



(2) 10 (^ of the same tissue \ias placed in 200 cc of absolute alcohol and 

 left for about 15 hours at room temperature (30° - 32*^ C). "ftor filtering, 15 cc 

 of distilled water vias added, it -..nl;? heated at 100^ C to eycnorate the alcohol, 

 and after two hours was concentrated to 2.7 cc (3»7 V^ of drier) srlted tissue to 

 [Pa'^e I22j Ice). Results of these experiments (Preparations "c. 1, 2, and 4) 

 indicated a lethal dosage of 0,2 ^x of flesh for Preparation ''o. 2 and of 1,0 gr 

 for Preparation 'To, U (calculated for a nouse wei^hinc^ 15 tt)* 



(3) 200 cc of water was added to 10 ";r of the sa/ie tissue and it v.as left in 

 a refri'^arotor at / 10° C for 15 hours, ^^^fter filterin-^, the filtrate was heated 

 rt 100° C for about 4 hours and concentrated to 13 cc (0,77 f?r of tissue to 1 cc). 

 The results of this experiment (Freparation Ho. 3) did not indicate the lethal 

 dosa'»e but it appeared to be rrrenter than 0„23 T of tissue, and, .ludf^in^^ by the 

 symptoms exhibited, it is probably more than tv.lce that anoimt. 



CTables for Preparations Tlos, 1 and 2 J 

 [Pape 123] [Tables for Preparations Mos. 2', 3, 43 

 LPa^e 124] [Tables for Preparations Nos. 5, 6, and 7] 

 [Paf^e 125] [Tables for Preparations 'los. 8, 9, and 10 J 

 [Pa*^e 126] [Two tables of experiments on cats] 



63 



