190fi.] NATURAL SCIEXCES OF PHILADELPHIA. 315 



on the effects of different killing fluids on proteid bodies enable us 

 to decide this question v,-ith a high degree of probability. He 

 classes albumoses among the " Granulabildner " — that is to say, 

 they are precipitated in the form of granules insoluble in water by 

 tannin, chromic acid, sublimate, platinic-chloride, formal- 

 dehyde, osmic-acetic, Fleraming's and Hermann's mixtures; and 

 they are precipitated in the form of granules soluble in water by 

 alcohol, acetone, picric acid, picric acid-alcohol, picric-sulphuric 

 acid {loc. cit., p. 33). The fixation method of determining the 

 presence of albumose would then be: (a) Precipitation with some 

 one of each of the two classes of fixatives (Fischer recommends 

 osmic-acetic and Hermann's for the first, and alcohol and picric 

 acid for the second); (6) washing both in water; (c) staining 

 with some dye which has a strong affinity for albumose (Fischer 

 recommends Altmann's acid-fuchsine-picric-alcohol). If albu- 

 mose is present it will be found in the first case, and will be washed 

 out in the second. Figs. 1 and 2, Plate XVI, are from the same 

 region of two intestines fixed twenty-four hours after feeding, the 

 former in Hermann's fluid, the latter in alcohol; both were washed 

 a long time in water (the former twenty-four hours, the latter 

 fifteen) ; they were carried through the same reagents together, and 

 finally stained in precisely the same manner, namely, in 15 per 

 cent, acid-fuchsine in aniline water for five minutes at 54° C, 

 then differentiated in picric-alcohol. (The effect of the picric- 

 alcohol cannot be shown in the figure. ) Figs. 3 and 4, Plate 

 XVI, are from intestines treated as above after fifty hours from 

 time of feeding. 



In order to test Fischer's results more fully, I have performed a 

 number of experiments similar to his on proteids obtained directly 

 from Griibler's Laboratorium in Dresden. A summary of these 

 experiments is given in Table II. It will be seen that my results 

 confirm Fischer's in most respects essential to the precijntation of 

 albumose. There is but one noteworthy difference, namely, that 

 whereas Fischer obtained from Griibler's heniialbumose (prot- 

 albumose) a granular precipitate essentially like that from deutero- 

 albumose, I find only coagulum by HgCl.^. " 



Peptone (depur. sice, aus Fibrine, Griibler) seems to be a mix- 

 ture of albumoses and true peptones. It is altogether likely that 



^1 Several other fluids gave the same. 



