606 PROCEEDINGS OF THE ACADEMY OF [Sept., 



colony king. With this worker I put another, fifteen days old, 

 whose parents were both of the C colony. The two affiliated at 

 once. 



Experiment b. — May 10. Into a Petri cell occupied by three 

 workers, from seven to twelve days old, the progeny of a C colony 

 queen and an H colony king, I introduced one by one three 

 workers, from two to twelve days old, the progeny of a C colony 

 queen and a C colony king. The affiliation was in each case 

 immediate. They behaved toward each other as if they had 

 always been associated. I then put the six young ants into an 

 artificial nest inhabited by queens and Avorkers of the C colony, 

 and they were adopted without examination. 



Experiment d. — June 11. Into a Petri cell occupied by five 

 workers, from two to five weeks old, the progeny of a C colony 

 queen and a C colony king, and engaged in the care of introduced 

 larvse, I put a worker a few days old, the offspring of a C colony 

 queen and an E colony king. The callow Avas adopted into the 

 group without objection of any sort. 



Experiment e. — June 11. Into a Petri cell occupied by six 

 workers, between one and three months old, the progeny of a C 

 colony queen and a C colony king, and engrossed in the care of intro- 

 duced larvse, I put a callow a few days old, the offspring of a 

 G colony queen and an H colony king. The callow was immedi- 

 ately accepted by the residents. 



Experiment f. — August 5. Into a Petri cell I put a callow two 

 days old, the offspring of colony parents, and hatched in one of 

 my artificial nests that had been established just one year. With 

 this callow I put one of about the same age hatched in the ances- 

 tral wild nest of the same colony. The two callows instantly affi- 

 liated, though they must have had different fathers. 



The following experiments show not only that the mother alone 

 determines the intrinsic odor of her offspring, but that the heredi- 

 tary odor is modified by the age of the ant. As in the foregoing 

 experiments, the workers first i)laced in the Petri cells are those 

 sequestered while in the pupa stage, having no acquaintance with 

 any ant other than those of their own segregated group of workers. 



Experiment g. — February 23. Into a Petri cell occupied by two 

 workers about one month old, the progeny of a C colony queen 

 and a C colony king, I put two large and dark-colored workers 



