1902.] NATURAL SCIENCES OF PHILADELPHIA. 767 



It is my purpose in this section to give an account of a process of 

 budding in a medusa very different from Cunma, one in which 

 the asexual multiplication takes place very differently. In Gonio- 

 nema the buds are produced in a manner which reminds one very 

 strongly <jf the similar process in Cassiopea. 



In the course of my general study of the development of 

 Gonionema I came upon the budding larva (text figs. 2-10). From 

 a lot of eggs obtained at Woods Hole, in August, 1901, a large 

 number of polyps developed and were kept alive in a balanced 

 aquarium for several months. This lot was left at Woods Hole in 

 as nearly natural conditions as possible until the last of November. 

 The water was kept fresh by frequently renewed supplies of dia- 

 toms and ulva, and occasionally changed by carefully adding a 

 quantity taken from the natural habitat of the medusa in the eel- 



Fig. 2. 

 Five months old polyp with bud just forming. 



pond. A low temperature was maintained. When these larvae 

 were received from Woods Hole (November 28) they were appar- 

 ently thriving well. They had all settled upon the Minot watch- 

 glasses which had been placed in the bottoms of the dishes. These 

 were easily removed without disturbing their contents. The watch- 

 glasses were numbered and the positions of the polyps carefully 

 noted and mapped. Successive examinations showed that the 

 number of polyps was increasing, and on December 3 it was seen 

 that one or two of the largest specimens had rounded knob-like 

 bodies upon them; these were at once recognized as buds. The 

 specimens were examined as frequently as it was thought safe to 

 remove them from the jar, and camera drawings were made of the 

 growing buds. Observations were made of the different stages in 

 the development of fourteen buds ; their phases agreed in all the 

 main particulars. 



