1913} YAMANOUCHI—ZANARDINIA II 
During these mitoses, chromatin globules accumulating outside 
of the membrane of the resting nucleus, and their gradual dis- 
appearance with the simultaneous increase of chromatin sub- 
stances within the network, are beautifully shown. In prophase, 
in metaphase viewed from the pole, and in 
anaphase, 22 chromosomes are distinctly 
shown (figs. 11-14) proceeding toward the 
pole with equal rapidity. 
The female gametangium comprising 
3-9 tiers of cells, with 2 or 4 cells in each 
tier, is now established. Each individual 
cell of the gametangium is a mother cel] 
and the whole contents of the cell become 
transformed into a single female gamete. 
The mother cell contains a large resting 
nucleus, surrounded by plastids. A por- 
tion of one of the plastids lying near the 
nucleus shows a deep orange color, which 
is the red pigment (fig. 14, a, b, c). When 
the female gametes are matured, a portion 
of the membrane of the mother cell dis- 
solves, forming a pore through which the 
gamete is discharged. The cilia of the 
gamete first appear outside the pore, keep waving for a time, and 
then the gamete is set free. 

Fic. 8.—Mature male 
gametangium after escape 
of gametes. 
Fertilization and germination of the fertilized female gamete 
The discharge of both male and female gametes occurs at almost 
any time during the day and night. Taking the case of a certain 
individual plant growing in a tank in the laboratory, the male 
gametangia matured and the discharge of the gametes began while 
the female gametangia were still in an immature condition, and did 
not mature and discharge their gametes until two or three days later. 
The time relations of the maturity of the male and female gametes 
_in nature may be similar to that in artificial cultures. A periodic 
discharge of the gametes from the mature gametangia was observed 
to be most abundant at about 5:30 A.M., after which the discharge 
