1913] YAMANOUCHI—ZANARDINIA 13 
membrane of the body, which now becomes spherical. The nucleus, 
with a very delicate membrane, shows a number of chromatin knots 
identical with the number of chromosomes (fig. 25). It is a notice- 
able fact that the nucleus 
of the male gamete, during 
- the period of active move- 
ment, is in the resting con- 
dition, and when the gamete 
becomes quiescent without 
any union with a female 
gamete, it still shows the 
Same structure as if it had 
united with the female 
gamete. ; 
When the male gamete has just bécome attached to the female 
gamete, both gametes have very delicate plasma membranes. 
The nucleus of the female 
gamete is at the center of 
the cell, as in the resting 
condition, but that of the 
male gamete shows 22 in- 
dependent chromosomes. 
The plasma membranes 
which lie between the 
cytoplasm of the male and 
female gametes become ob- 
seure and the cytoplasm of 
the two gametes comes into 
direct contact (fig. 27). 
The body of the male 
gamete can be observed 
for a short period as a pro- 
of chromatin globules around the outside of the tuberance from that of the 
pie ee in anaphase and the female gamete (fig. 27). 
Later, the protuberance is 
less prominent (fig. 28), and finally it is leveled down to the 
spherical curve of the body of the united gametes (fig. 29). The 

Fic. 10.—Female gametangia: nuclei in the 
first division. 

