1913] YAMANOUCHI—ZANARDINIA 27 
such blepharoplasts or centrosomes. That the granule which is 
the blepharoplast primordium arises in the cytoplasm, and after- 
ward becomes established as the blepharoplast, shows physiological 
connection with the nucleus holds true in Polytoma, Hydrodictyon, 
Derbesia, Cutleria, and Zanardinia. 
The zoospore in the free swimming condition is oval (fig. 23, a, b) 
and usually contains more than 30 plastids. A portion of one of 
the plastids near the blepharoplast, which lies in the plasma 
membrane, produces a deep orange color which is the red pigment. 
The length of the zoospore is 22.5 y; the cilium directed toward 
the anterior end is 2 times the length of the zoospore, and the 
other has the same length as the zoospore. 
Germination of zoospore 
The zoospores were observed to continue in the motile condi- 
tion at the longest 2 hours and at the shortest only 10 minutes. 
Toward the end of the movement, the zoospore becomes sluggish, 
its body gradually assumes the spherical form, and by this time 
the cilia become tangled and coalescent with the plasma membrane. 
The formation of the cell wall upon the plasma b is gradual. 
About 4 hours after the zoospores have become quiescent, no wall 
has yet been formed (fig. 64). In a majority of cases, about 20 
hours after quiescence a delicate cell wall is first recognized (fig. 65). 
The first segmentation mitosis of the germinating zoospore 
takes place about 24 hours after quiescence. The nucleus enters 
prophase at 24 hours, but the metaphase stage is found only in 
the material fixed 26 hours after quiescence (figs. 67, 68). The 
number of chromosomes counted at metaphase in polar view is 
22, the reduced number (fig. 69). Anaphase and telophase imme- 
diately follow metaphase (fig. 70) and the sporeling reaches the 
2-celled stage. One of the two cells in the sporeling, which is 
derived from the elongated portion of the sporeling at the 1-celled 
Stage, either divides once (fig. 72) or remains undivided and becomes 
the holdfast; while the other cell continues to divide (figs. 71. 73, 
74). Some of the sporelings up to the 5-celled stage, obtained in 
cultures after 2 or 3 days, are shown in fig. 24. In about 25 days 
the sporeling has developed into a long filament (fig. 18, 6).. Later 

