
118 BOTANICAL GAZETTE [AUGUST 
under bell jars on the laboratory table. Parchment paper proved 
unsatisfactory because its opaqueness and fibrous character made 
observation difficult; so after several sets were run with no 
germination, hog bladder was substituted. After a few trials good 
TABLE It 
SHOWING THE EFFECTS OF VARIOUS SOLUTIONS ON THE POLLEN OF Trifolium pratense 

Solutions 


Sucrose 0.731 +KNO, 0.00006.| Bursting 





Sucrose 0.731+KNO; 0.0002..| About 25 per| 75 per cent No germination 
cent bursting} turgid 
Sucrose 0.731+KNO; 0.0006..} About 25 per| 75 per cent : . 
cent bursting} turgid a 
Sucrose 0.731+KNO, 0.002. .| Very little Turgid Z 
bursting 
Sucrose 0.731+KNO; 0.005. ..| No bursting % . - 
prea o/31 galactose ‘.. 3888 Very little r : 
+ a trace of asparagin...... bursting 
Sucrose a etna 
ete We Pits Loe Bursting “é 
hoe 3S. ee ripa ag 1.3888] Little bursting) 25 per cent or} “ 
BNO) G:0008. Soci. ss more turgid : 
Sucrose o. 731 sith 0075 aspara-| About 50 per] 50 per cent iz 
MA ea. cent bursting turgid 
rppicnbesl oO. He oees Fee ry eee YO eee 
asparagin 0.075... ........ 
Sucrose 1.02 255-Fasparagin 0.15 Me eee fea vke Lt eet 
Sucrose 0.8772+galact Rie See PRS CW saree OL > eens eee 
1.9333-+asparagin o, 
Sucrose 0.8772 ar yabatonth = 
etre) Cle acy os No burstin Turgid . 
Sucrose 0.8772+asparagin . % 
SE OSS 8 BOC OS 8 Pie 8 bk ee va 3 . * ¢ 
Sucrose °. ;8772-Tasparagin 
PAP OS 618 8 Od oe e686 Se 8 - . : : 
Sucrose °. tern oleic 
PN Rie ey A Ae ale ie cee alli. egg Seg ig Ik! as ee sd : 
Suc ae 0.8772+palmitic acid..|  “ . a . ; 
bcos 0.8772 Bee oe ee er « « « “ : 
Pevulose 10004-lipase. 1. ces * : ‘ 
thin+H,0 asa thick eee SS ® % r . 
Street as a thick 
SMR e O MR et g hgs be gh yas ag! “ . : ‘ 
inion ahi as a thick 
Mee eS . . : 
Sucrose 0.8772+diastase...... . « . . e 



















germination was obtained on the bladder. It was found that 
germination was very closely connected with the amount of water 
in the membrane, and it was not easy to dry the membrane 
So as always to secure germination. Membranes soaked in 
