260 BOTANICAL GAZETTE [OCTOBER 
are significant. The method of counting was probably not strictly 
accurate, and, furthermore, the difference in the times of taking 
samples might well affect the absolute numbers. It is also true 
that the investigation of a larger number of samples would be 
desirable before drawing final conclusions. Nevertheless, the 
comparative results indicate that the fungi in the soil investigated 
were distributed rather uniformly at different depths, at least as 
low as 14cm. No samples were taken at a greater depth. This 
result is quite different from that usually found for bacteria (see 
Kine and Doryanp 35). 
Some study was also made of the depths at which particular 
fungi were found. From the results obtained, no definite rela- 
tionship was discovered. Any particular species seemed about as 
abundant at one depth as at another, down as deep as samples were 
taken. The results for the three most abundant species are given 
in table III. 





TABLE III 
Toraz NUMBER OF CULTURES AT EACH DEPTH 
Name NUMBER OF 
ISOLATIONS acm. | 4cm.| 8cm. | r2cm.| 14cm. 
Se eee: 
Pomcum (5.2)... 13 2 I 5 3 : 
Mucor ambiguus............... 14 4 3 I 3 3 
Trichoderma nigro-virens...... . 8 3 4 ° ° 
ESE aaa 






These data were obtained, not from the original plate cultures, 
but only from the pure cultures isolated from the plates. This was 
for the reason that the fungi were many of them not identified until 
after they had been isolated for some time, when those in the plates 
had overgrown each other too far to be distinguishable. The data 
here given are tegarded as insufficient for the drawing of final 
conclusions, but as far as they go, they do not seem to show any 
spat relationship between the position of particular species and 
epth. a 
DISTRIBUTION ACCORDING TO TREATMENT OF SOIL 
As previously Stated, the three plats were differently treated 
as to tillage and fertilization. It was expected that a marked 
difference would be found in the flora of the different plats, espe- 
