1913] GODDARD—SOIL FUNGI 283 
in the form of the dry salt for the test analyses, it was found neces- 
sary to have the salt thoroughly powdered before adding the 
sulphuric-salicylic mixture. In nearly all cases the analyses were 
carried through in duplicate. 
By both methods of analysis used, a series was carried through 
with some substance of known composition and purity, in order to 
make sure of accurate results from the method. Asparagin was 
used for the nitrate-free method, while ammonium nitrate was 
employed for the method including nitrates. For all small amounts 
of asparagin, a suitably large quantity of the pure salt was carefully 
weighed on an analytical balance and then dissolved in a quantity 
of water measured up in a volumetric flask. For each analysis, 
the desired quantity of this solution was measured off into an 
evaporating dish by use of an accurate burette. The solution was 
then evaporated down to 5-7 cc., and was then poured into the 
Kjeldahl flask, together with two or three small rinsings. The 
following tabulation gives the results of these analyses in compara- 
tive view: 
TABLE IV 
Blank determination gave N 0.42 mg.; calculated per cent of N in asparagin, 
18.70; solution 1, 80 mg. asparagin dissolved in 200 cc. distilled water; solution 2, 
300 mg. asparagin dissolved in 1000 cc. distilled water. 








N obtained | Calculated | Difference | porcentage Variation 
No. Material used ee —_ ee ie Name from brea 
a b € d € 
Ea 100 tng, dry salt... .:.. 17.97 18.70 0.73 17.97 0.73 
Ree oe ere 18.11 18.70 0.59 18.11 | —0.59 
2 on Aan agate ee ren eg ee 18.04 18.70 0.66 | 18.04 | — 
ee es ees 8.98 9.35 0.37. | 17.96 | —0.74 
Bt ee eae ey 8.08 9.35 0.37. | 17-96 | —0.74 
ee ec ins 8.92 .35 0.43 | 17.83 | —0.87 
oe ee is 3.79 3-74 0.05 18.90 20 
8] 20 “ (socc.sol. 1)... 3.65 406 0.09 18.25 | —0.45 
O71 3S — (e66c, tol 2) ...| 3.67 2.80 0.13 17.80 | —0.90 
To} 15 “ (duplicate of 9)..| 2.70 2.80 ©.10 18.00 | —0.70 
nile epee ae a 0.56 OG fos iss Heese ees 
12) 3 “ (duplicate of 11) | 0.49 0.56 Oe ee Pl ae Ea 

The similar analyses were not run in duplicate except as indi- 
cated. It will be seen that the greatest variation occurs in the case 
of no. 7, where a rélatively small quantity of dry salt was used. 
