286 BOTANICAL GAZETTE [OCTOBER 
Fusarium (sp. ?), Acrostalagmus cinnabarinus, Pachybasium hamatum, 
Aspergillus calyptratus, Hormodendron cladosporioides, Monilia 
Koningi, Stysanus stemonites, and Trichoderma nigro-virens. Four 
inoculations were made with each fungus, two in N-free cultures 
and two in ammonium nitrate cultures. This gave duplicates 
of all cultures. Growth took place in all of these, except those 
of Stysanus and Hormodendron. One of the duplicates for each of 
these failed to show any germination, and evidence was obtained 
later that the spores were no longer viable. In one or two cases 
there were doubts about the purity of cultures. On these accounts, 
it was decided to make another trial of the whole set. This result 
should be noted, however, that while a visible amount of growth 
took place in all these cultures, the amount was extremely small 
in all N-free cultures, and furthermore, it all took place within two 
or three weeks, after which no growth was to be observed, although 
observations were continued for over three months. 
INVESTIGATION II 
The second investigation was carried out along the same general 
lines as the first, but with several modifications. First, Erlen- 
meyer flasks of about 150 cc. capacity were substituted for the test 
tubes. In each flask was placed socc. of the culture medium 
instead of 25 cc. as before. Second, the culture solution was modi- 
fied, using a higher percentage of phosphate and nearly doubling the 
amount of sugar, which was supplied this time in the form of 
dextrose. The full formula was as follows: 
mer ee 1000.00 CC. 
WO ee 30.00 gm. 
Monopotassium Create eo Pe 2.00 gm. 
PRRRUEAIOIA SUIGIALE: ic 2 ec i oo o. 20 gm. 
ee Oloasie oe, 0.10 gm. 
tite. ee 0.02 gm 
For controls 
Moreen inte 2. 2.00 gm. 
This was a medium which had been found well adapted to luxuri- 
earths growth in agar and gelatin cultures. Third, 14 species of 
fungi were used for the inoculations, including all in the complete 
