1913] GODDARD—SOIL FUNGI 287 
list except Mucor stolonifer, Penicillium glaucum, P. bicolor, Stysanus 
stemonites, and Trichoderma Koningi. New cultures had been 
made of all of these and it was made certain that the spores were all 
viable and the cultures all pure. Inoculations were again in dupli- 
cate, giving four cultures of each fungus, two in N-free media and 
two in ammonium nitrate media. All the cultures were placed 
in the laboratory, protected from the air only by cotton stoppers. 
Observations were made once a week from January 3 to March 11, 
about 68 days. 
Good growth took place in all the cultures and there were good 
indications that all were pure according to the inoculations. This 
was shown first by the fact that the duplicates in every case were 
very closely similar. Second, each culture showed the peculiar 
culture characters belonging to the species, such as coloring the 
medium pink in the case of Fusarium, or the black mycelium in 
the case of Hormodendron. Third, the four that were afterward 
analyzed were submitted to microscopic examination, which showed 
characteristic spores and fructifications, although spores were only 
meagerly produced in the N-free cultures. The amount of growth 
in the N-free cultures as compared with that in the ammonium 
nitrate cultures, as it appeared to the eye, is shown in fig. 17. 
The mycelium in the former was very loose and flocculent and 
almost wholly submerged. To the eye, it appeared to spread 
through two-thirds of the liquid in some cases, but as will be seen 
in the analytical results, when dried its weight was extremely 
small. It is worthy of note also that this growth all took place 
within the first 2 or 3 weeks, after which it ceased entirely. The 
gtowth in the ammonium nitrate medium was very abundant. 
The mycelium soon reached the surface, where spores began to 
form abundantly. The growth continued for many weeks and 
finally resulted in a thick felt over the surface and a mass of 
mycelium filling the liquid. 
Five of these cultures which gave signs of the largest growth in 
the N-free solutions were now taken for analytical examinations. 
The mycelium of each was collected on a dried filter and dried to 
4 constant weight. The filtrate was evaporated as previously 
described and analyses were made of both mycelium and filtrate. 
