1913] ° GODDARD—SOIL FUNGI 289 
Table VI shows the results of this set of analyses. The nitrogen 
of a blank determination has been subtracted in each case from the 
total nitrogen obtained. 
TABLE VI 
DEVELOPMENT PERIOD 68 DAYS 

Dry wt. of | N of Not | Total N of fixed 
myosin mycelium trate culture 
No. Fungus mg. msg. mg. — 


rt | Control inoculated with 
mevcoiomthora «5 ee. 13 a A ©.49 0.49 |...-.. bas 
Control inoculated with 
ndro 
nN 


Spends See ees ee 0.42 O.4F aS. 
re MC oS 0.45 OAS tas 5 
3 pathos Sit koe 6.0 0.49 0.42 0.o1 0.46 
4 | Hormodendron......... wx 0.42 0.28 0.70 25 
5 | H ormedendron (dup.).. 9-4 0.07 0.21 0.28 | —0.17 
6 | Pachybasium........... 5.0 0.14 0.42 0.56 o.1I 
7 Bich Visi fap.) os 5.6 0.07 0.42 °.49 04 
8 | Acrostalagmus.......... 8.6 0.28 0.14 0.42 —0.03 
9|/A seg (dup.):... 9.0 0.21 0. 28 0.49 0.04 
oe ee ee 6.0 0.07 0.35 0.42 | —9.03 
11} F eacan ome)... 82S. 4.2 0.07 0.07 0.14 | —0.31 








From later work, it was believed that the dry weights of myce- 
lium, given in column a of the table above, were a little too large. 
The reason for this seemed to be that the mycelia were not washed 
quite long enough to remove every trace of dextrose. Again, the 
nitrogen fixed, as indicated in column e, was high enough for nos. 
3 and 4 to raise the question whether these two fungi might possibly 
have a little nitrogen-fixing power. To test these points further, 
cultures of these two fungi were run at the same time with later 
investigations, extending from April 17 to June 26. These cultures 
were treated exactly as in the above series, except that the phos- 
phate content was increased to 3.0 gm., and the dextrose content 
to 45.0 gm. in 1000 cc. of the solution. This gave a higher nitrogen 
content for the controls, as well as for the filtrates of the cultures, 
since the glucose was found to contain some nitrogen. In washing 
the mycelia, 200 cc. of water was used instead of 100 cc. as in the 
earlier work. Table VII gives the results of these tests. 
