1913] GODDARD—SOIL FUNGI 205 
was made in which exactly 16 gm. of the C.P. salt were dissolved 
in ammonium-free water. The salt for this was of course weighed 
out with the greatest accuracy on the analytical balance. The 
solution was then made up to exactly 1000 cc. in a volumetric 
flask, and was called solution B. Cultures were all made in dupli- 
cate. First, 25 cc. from solution A and 25 cc. from solution B 
were drawn off, by the use of an accurate burette, into duplicate 
flasks. This gave 50 cc. cultures, each of which contained exactly 
0.40 gm. of pure ammonium nitrate, or approximately an M/1o 
solution. Then socc. of solution A were accurately drawn off 
into a volumetric flask of 250 cc. capacity. This flask was then 
- filled to the mark with ammonia-free water, thus giving a solution 


TABLE IX 
CONTROL, 100 CC., STERILIZED AFTER INOCULATION, 1.32 MG. N 
Dry = of N ~ ad of — Lom “ Gain 
No. mycelium | mycelium | less contro! —c¢ 
0. Name of fungus ng iar: frgeo ing. mg. 
a b ¢ d e 
EAE BUNNTTA rie ee ReaD eee NEON a 
1 | Myceliophthora......... 3.0 0.07 0.08 | 0.15 0.15 






3 
Sugty ity Vhs oe 1.2 
4 sarium (non-conduc- Not analysed 
tivity water). ........ io 
Si Pachybasiom. .....;.... 1:6 

of § the strength of solution B. Two culture solutions were now 
made by accurately drawing off 25 cc. of this diluted solution of 
ammonium nitrate and 2 5 cc. of solution A into culture flasks. 
This gave an M/ 50 solution of ammonium nitrate. By the con- 
tinuation of this dilution process, further culture solutions were 
made containing M/250, M/ 1250, and M/62s50 respectively of 
ammonium nitrate, all solutions having the same quantities of the 
other constituents as in investigation III. This may all be seen 
in brief in table X. 
These cultures were sterilized and inoculated in the usual way 
and then allowed to stand in the laboratory without special pro- 
_ tection from the air. During the final sterilization, a slight change 
