1913] GODDARD—SOIL FUNGI 207 
liquid itself was made yellowish brown. In the three higher con- 
centrations a thick botryoidal mat was developed. 
At the end of the growth period, all of the cultures of Myceli- 
ophthora were submitted to analysis to determine the amount of 
nitrogen in the mycelium and in the filtrate. The M/s50 culture 
solution was analyzed for nitrogen content as a check on the purity 
of the ammonium nitrate. The medium without ammonium 
nitrate was also analyzed. The mycelium in each case was ana- 
lyzed by the nitrate-free method, while the nitrate method was 
used for all the filtrates. The results are all incorporated in 
table XT. 









TABLE XI 
DEVELOPMENT PERIOD 48 DAYS 
Dry wt. of | Ninthe | .Ninthe | TotalN | N supplied | 7 gain 
No.| Concentration 4 age —" medium (12) — - —_—. mg. 
mg. 
a b ¢ d e f 
First series > 
. Mie wet: 98.0 5.41 | 130.85 | 136.26 | 140.19 | 3-94 
2 Pees pk 148.1 7.51 20 27.59 28.04 | —0.45 
“fy ie dds Bae ee 242.6 4-91 0.70 5.6 5.61 0.01 
er Mra: 7 1.40 | —o.14 1.26 1.12 0.14 
$1: /G2g0...... .. 26.6 0.70 | —o.21 ©.49 0.22 0.27 
Duplicate series 
a RE Ee 08.6 5-19 | 130.71 | 135.90 | 140.19 | —4.-29 
eg) | 0 Sia 166.6 75 19.66 | 27.24 | 28.04 | —0.80 
C1 Mim. . 20... 257.2 5.19 2 5-62 5-61 Os 
9 | M/t250...... 76. 1.26 0.00 1.26 1.12 0.14 
10 | M/6250...... 25.4 0.42 0.00 0.42 0.22 ota 
tt | M/s0 control, not inoculated, 27.80 mg. N. 
12 | Medium without NH,NO; (50 cc.), 0. 70 mg. N. 


It is difficult to find any evidence whatever of nitrogen-fixation 
in these results, even though the combined nitrogen enabled the 
fungus to begin a vigorous growth. The nearest suggestion of 
evidence appears in analyses 4 and 5, where the mycelium seems 
to have gained a slight amount of nitrogen. In the same analyses 
the filtrates show a slight loss. Whether there is significance in 
this or whether it is just a coincidence in the errors of two analyses 
Seems hard to say, since the amounts are so small as to give one 
little confidence in their importance. Furthermore, the duplicate 
