188 BOTANICAL GAZETTE [MARCH 
from the mitotic figure, or karyokinesis might vary in this par- 
ticular point on account of the many varieties of the cultivated 
hyacinth. 
This persistence of the nucleoli after the formation of the 
achromatic figure, which Rosen found in only a small portion of 
his hyacinth material, I found in all the material examined, from 
tips of Allium just started within the bulb and from those grown 
in water or solutions for some time. The duration of the nucle- 
oli varies. I have never seen any signs of the presence of the 
nucleolus as a stainable body in the spindle space or in the cyto- 
plasm after the daughter chromosomes have begun their move- 
ment toward the poles. figs. 2-30 show the condition of the 
nucleoli as the formation of the achromatic figure advances; figs. 
36, 37 are chosen from the many cases observed showing their 
dissolution in the cytoplasm. These go to prove that in Allium 
at least the nucleoli do not furnish to any appreciable degree 
the material for the achromatic figure, as Strasburger (14), Hof 
(11), and Némec (9) have claimed. 
Neither did any cells yield evidence, such as Némec (9) gives 
in his work upon Allium, that the new nucleoli arise from the 
substances coming from the degeneration of the mantle fibers. 
The degenerating mantle fibers appear rather to be streams of 
granules which become diffused in the cytoplasm, to gather again 
at the line where the cell plate forms. In figs. 45 and 46 are 
two periblem cells in stages closely succeeding one another and 
approximating the time for the appearance of the nucleoli. No 
aggregations of granules which stain like nucleoli could be 
observed in these cells or in any similar stages. If we consider 
the mantle fibers and the like in the living cells as streams of albu- 
minoids and other substances issuing from the nuclear elements 
in their activity, then the apparent degeneration of the fibers 
merely indicates a diffusion of these dissolved substances and a 
lessening of their flow. These streams, after the chromosomes 
have arrived at the poles, are mainly directed in their course to 
the equatorial zone, separating the two daughter nuclei where the 
deposited substances lead to the building of the cell plate. 
As stated in the section upon the chromatic figure, at the time 
