glass bottles which contained 25 ml. of O.OIN 

 HCL. All containers used for sampling were 

 chemically cleaned prior to use. 



Water temperatures were measured with a 

 thermistor to the nearest tenth of a degree 

 centigrade. 



Light measurements (in microamps) were 

 made with deck cell and sea cell and recorded 

 as percentage of light penetration to the 

 nearest tenth of a percent. Surface photometer 

 readings were taken at approximately 2 feet 

 below the surface. Secchi disc readings were 

 also made and they were recorded to the 

 nearest one-half foot. 



Cloud type, cloud amount, sea state, visi- 

 bility, water color, and Secchi disc readings 

 were estimated by the observers and coded as 

 indicated in the section on the explanation of 

 column headings. Wind direction and sea 

 direction were given by points of the compass 

 and are accurate to -10°. 



All stations were sampled at high tide -1 

 hour, with the exception of the offshore sta- 

 tions 21A, 21B, 21, 23, 25, 27, 27A and 27B, 

 which were sampled without regard to the 

 tidal stage. 



Enumeration of Gymnodinium breve 



Counts of G. breve were made according to 

 the enumeration method decribed by Drago- 

 vich, Finucane, and May (1961). 



Chemical Analysis 



Salinities were determined by the Mohr- 

 Knudsen method (Knudsen, 1901). 



Nitrate- nitrite nitrogen determinations were 

 made by the method of Zwicker and Robinson 

 (1944) as modified by Marvin (1955b). Due to 

 the formation of color in the blanks employed 

 in the NO3 -NO2 analysis, the accuracy of the 

 method for the low ranges encountered was 

 found to be to.2/ig.at./l. of NO3-NO2-N. 



Ammonia determinations were made ac- 

 cording to the Wirth and Robinson (1933) 

 method. Since a number of substances inter- 

 fere with the Nessler reaction when ammonia 



concentrations are low, accuracy better than 

 to.l to 0.2//g.at./l. cannot be attained in the 

 range measured here. 



Inorganic nitrogen was distilled as ammonia 

 from an alkaline solution and measured col- 

 orimetrically using sodium phenate as rea- 

 gent. After digestion with sulfuric acid, the 

 residue was again made alkaline and the 

 organic nitrogen was acquired and measured 

 the same as for the inorganic determination. 

 No samples were filtered prior to analysis. 

 This procedure was developed as a micro- 

 analytical method by Willis. 1 



Calcium determinations were made accord- 

 ing to the de Sousa (1954) method. 



Alkalinity measurements were made by the 

 method of Thompson and Anderson (1940). 



The Harvey (1948) method was used for 

 determinations of total phosphate-phosphorus 

 and the method of Robinson and Thompson 

 (1948) was used in inorganic phosphate-phos- 

 phorus determinations. 



Concentrations of silicon were determined 

 by the Armstrong (1951) method. 



Some of the phosphorus, silicon, and total 

 organic nitrogen values are listed as greater 

 than a given value. This notation indicates that 

 the field sample value exceeded the upper 

 limit of the calibration curve. The given value 

 represents the upper limit of the particular 

 calibration curve established at the time of 

 the determination. 



None of the samples were filtered prior to 

 the chemical analyses. 



ACKNOWLEDGMENT 



McKinley W. Jambor and Lucius Johnson 

 assisted in the enumeration of G. breve and 

 chemical analyses respectively. 



Robert M. Ingle and Robert F. Hutton from 

 the Florida State Board of Conservation coop- 

 erated by exchange of red tide information. 



1 David C. Willis. 1960. A microanalytical method 

 for the analysis of total nitrogen in sea water. (Type- 

 written manuscript on file at the University of Tampa.) 



