Figure 2. --Tampa Bay area showing station locations. 



METHODS 



Sampling Techniques 



The entire sampling was conducted from 

 the research vessel Kingfish, a 43-foot, twin- 

 screw, diesel cruiser, equipped with an echo 

 sounder and radar. 



Water samples were collected with modified 

 Van Dorn sampling bottles (Van Dorn, 1957) 

 from three or four evenly spaced depths, in- 

 cluding surface and bottom. Samples for the 

 determination of ammonia and total and inor- 

 ganic nitrogen were collected at selected sta- 

 tions and from the surface only. Samples for 

 total phosphate -phosphorus, inorganic phos- 

 phate-phosphorus, nitrate-nitrite nitrogen. 



and ammonia were immediately transferred 

 from the sampling containers into 200-mm. 

 culture vials which were capped with poly- 

 ethylene-lined screw caps and quickly frozen. 

 Samples for the determination of total in- 

 organic and organic nitrogen were transferred 

 into 250-ml. glass containers, quickly frozen 

 and also capped with polyethylene-lined screw 

 caps. Samples for the determination of calcium 

 were transferred into 250-ml. glass-stoppered 

 bottles. Samples for the determination of sili- 

 con were transferred into 125-ml. polyethylene 

 containers with polyethylene screw caps. 

 Samples for salinity determinations were 

 transferred into 4-ounce glass prescription 

 bottles and for alkalinity measurements 100- 

 ml. samples were transferred into 250-ml. 



