

<•»>•> 





Fij^ire 3 



Figiire k 



• I* » ^«-,» ■ V - 



»>. .*>}■ ' ;, 



FigTire 7 



Explanation of Figui-es 



Photomicrographs of the intestine of li-week trout fry (yolk sac 

 still present) and adult guppies; fixed in Bouin's fluid, embed- 

 ded in paraffin, sectioned at 8 ji and stained in Hematoxylin- 

 Eosin. X li35 



Figure 3 — Intestine of normal guppy showing well-defined columnar 

 epithelium and connective tissue. 



Figure h — Intestine of guppy in .032 ppm DDT for two days showing 

 disorganization and necrosis of columnar epithelium and 

 connective tissue. Nuclei of the epithelial layer ap- 

 ppear indistinguishable from nuclei of the connective 

 tissue. 



Figure S — Intestine of normal trout fry showing organized columnar 

 epithelium, a few goblet cells, and normal connective 

 tissue. 



Figure 6 — Intestine of trout fry in 10 ppm DDT for lii days showing 

 absence of connective tissue below columnar epithelium 

 in parts. Necrosis of remaining connective tissue in 

 villus is marked. 



Figure 7 — Intestine of trout fry in 3.2 ppm DDT for Ih days showing 

 severe vacuolation and absence of organization of colum- 

 nar epithelium. Connective tissue did not appear to be 

 affected in this trout. 



20 



