t:M ' -i 



Fij'tire 8 



Figure 9 



•« i'^ 



-^^py^ 





Fisiire 10 



Figure 11 



Explanation of FigTores 



Photomicrographs of the liver of U-week trout fry (yolk sac still 

 present) and adult guppies; fixed in Bouin's fluid, embedded in 

 paraffin, sectioned at 8 >i and stained in Hematoxylin-Eosin. 



Figure 8 — Liver of normal guppy in which liver cells are solidly 

 packed . X lU? 



Figure 9 — Liver of guppy in .032 ppm DDT for one day showing foamy 

 appearance due to severe vacuolation and necrosis of en- 

 tire liver. X li;? 



Figure 10 — Liver of normal trout fry showing even distribution of 

 small vacuoles, perhaps fat vacuoles resulting from re- 

 sorption of the fatty yolk. Nuclei all appear to be 

 about the same size. X 14.35 



Figure 11— Liver of trout fry in 3-2 ppm DDT for one day shewing 

 vacuoles as in the normal though some are possibly 

 larger. Large irregularly swollen nuclei are scattered 

 throughout the liver. X k3^ 



21 



