Figuro 12 



Figure 13 



FiLTire lU 



Fi,":ure 1$ 



?i"iu'e 16 



Explanation of Figures 



Photomicrographs of the kidney tissue including tubules and adre- 

 nal tissue of U-week trout fry (yolk sac still present) and adult 

 guppies; fixed in Bouin's fluid, embedded in paraffin, sectioned 

 at 8 )i, and stained in Hematoxylin-Eosin. X U25 



Figure 12— Kidney of trout fry in 3.2 ppm DDT for lU days showing 

 congestion of tubules and necrosis of tubular epithe- 

 lium which is sloughing off into the lumen. 



Figure 13— Kidney of normal guppy showing solid whorl of epithelial 

 cells. This adrenal tissue was found scattered through- 

 out the kidney. 



Figure lU — Kidney tissue of guppy in .032 ppm DDT for two days show- 

 ing foamy appearance of whorls of epithelial cells which 

 are in a state of necrosis and vacuolation. 



Figure 15 — Kidney tissue of normal trout fry showing solidly packed 

 whorl of epithelial cells of adrenal tissue adjacent to 

 a kidney tubule. 



Figure 16--Kidney tissue of trout fry in 10 ppm DDT for lU days 



showing apparently healthy or normal epithelial cells, 

 identified as adrenal tissue, around the central blood 

 vessel. The disorganized appearance of these cells and 

 their position near the blood vessel indicate either 

 recovery of adrenal tissue or incomplete destruction 

 originally. 



22 



6 «57 



