hydrolysis of the phospholipids, by precip- 

 itation as the enneaiodide, conversion of 

 the iodide to iodate with bromine, and ti- 

 tration with sodium thiosulfate. Compar- 

 ison of Ba(0H)2 and methanolic HCl hydrol- 

 ysis techniques showed nearly identical 

 values with phospholipids of approximately 

 25% sphingomyelin content. With those of 

 over 50% sphingomyelin, the methanolic HCl 

 method gave values 10% higher. 



Fairbairn, D. 



1945. Free fatty acids in animal tissues. 

 _ Journal of Biological Chemistry , 157: 



• 645-650. 



A rapid loss of phospholipids occurs 

 shortly after tissue is removed due to 

 autolysis (approximately 8% in a few min- 

 utes in whole cat liver; approximately 15% 

 in ground liver). Homogenization in alco- 

 hol or fast-freezing decreases autolysis. 



Farvarger, P. and J. Gerlach 



1958 . A quantitative evaluation of 



Twitchell's method for the separation 

 . of saturated and unsaturated fatty acids. 



^ Archives des sciences (Geneva), II; 



* 539-542. Chemical Abstracts, 53; 

 19075h (1959). 



Twitchell's lead salt operation method 

 ( Journal of Industrial and Engineering 

 Chemistry , 13: 806, I92I) was used to 

 separate the fatty acids of the mouse into 

 saturated and unsaturated fractions . Re- 

 coveries were followed by addition of C 

 labelled fatty acids. Recoveries ranged 

 from 84.2 to 95.7%. 



of Clinical Pathology, 23: 599-602. 

 Cholesterol is precipitated as the digito- 

 nide, and the digitonide is quantitatively 

 determined by measurement of the color 

 produced with anthrone reagent. 



Feulgen, R . and H . Grunberg 



1939. Estimation of plasmal (plasma - 

 logen) in lipoid mixtures and in organs. 

 ^ Zeitschrift fiir physiologische Chemie , 



257: 161-172. Chemical Abstracts , 

 33:3407^ (1939). 

 Plasmalogens in lipid mixtures were de- 

 termined by photometric measurement of 

 the color produced with fuchsin-sulfurous 

 acid reagent. 



Feulgen, R., W. Boguth, and G . Anderson 



1951. Determination of acetal phospha- 

 tide (plasmalogen) in serum with regard 

 to the Waelsch effect. Zeitschrift fiir 

 ^ physiologische Chemie, 287: 90-108. 



Chemical Abstracts, 48: 462 Ig (1954). 

 Acetal phosphatide of serum is determin- 

 ed by hydrolysis with HCl in an acetic acid 

 solution, neutralization with NaOH, and de- 

 velopment of color with SO2 -reduced fuchsin. 



Fillerup, D. L. and J. F. Mead 



1953. Chromatographic separation of the 

 . plasma lipids . Proceedings of the Soci - 



^ ety for Experimental Biology and Medi- 



* cine, 83: 574-577. 



Plasma lipids were separated into groups 

 by chromatography on silicic acid with ethyl 

 ether -petroleum ether and methanol -ethyl 

 ether as eluting solvents. 



• 



▲ 



Faure, M. 



1950. Method for purification of lecithins. 

 Bulletin de la Societ§ de chimie biolog- 

 ique; 32: 503-508. Chemical Abstracts, 

 45:695F(I95I). 

 Lecithin was purified by precipitation 

 with CdCU. removal of Cd, precipitation 

 from ether with Me2C0, extraction with 

 ether, evaporation, and chromatography 

 of the ether solution on alumina . 



Feichtmeir, T. V. and J. Bergerman 



1953. Indirect colorimetric determina- 

 tion of cholesterol. American Journal 



Fink, K. and R. M. Fink 



1949. Application of filter paper parti- 

 tion chromatography to quantitative 

 4_ analysis of volatile and non-volatile 



_ organic acids. Proceedings of the Soci- 



ety for Experimental Biology and Medi- 

 cine, 70: 654-656. 

 The Cj-Cg acids are chromatographed on 

 filter paper as their potassium hydroxamates 

 and located on the developed chromatogram 

 by spraying with ferric chloride solution . 



Fiske, C . H . and Y . Subbarow 

 ▲ 1925. The colorimetric determination of 



20 



