serine component is removed by 30 hour 

 HCl hydrolysis and crystallization from ab- 

 solute alcohol. 



Folch, J. 



1942. Brain cephalin, a mixture of phos- 

 phatides. Separation from it of phos- 

 . phatidyl serine, phosphatidyl ethanol- 



amine, and a fraction containing an ino - 

 sitol phosphatide. Journal of Biological 

 Chemistry , 146: 35-43. 

 Phosphatidyl serine, phosphatidyl ethanol- 

 amine, and an inositol phosphatide were 

 separated by their differences in solubility 

 in chloroform and alcohol . 



Folch, J. and D. W. WooUey 



1942. Inositol, a constituent of brain 

 phosphatide. Journal of Biological 

 Chemistry, 142: 963-964. 

 Inositol is separated by precipitation and 

 HCl hydrolysis. Inositol is found in brain 

 in combined form . 



Folch, J. 



1948. The chemical structure of phos- 

 ■^ phatidyl serine. Journal of Biological 

 ▲ Chemistry , 174 : 439-450. 



A method is described for the isolation 

 of phosphatidyl serine of at least 92% puri- 

 ty by means of precipitation, dialysis, and 

 solvent recrystallization procedures. 



Folch, J. 



1949. Complete fractionation of brain 

 cephalin; isolation from it of phos- 



. phatidyl serine, phosphatidyl ethanol- 



amine, and diphosphoinositide . Jour- 

 nal of Biological Chemistry, 177 : 497- 

 504. 

 The cephalin fractions are separated by 

 their different solubilities in organic sol- 

 vents. 



washing with water . The method was com- 

 pared with those of Brante ( Acta physiolog- 

 ica Scandinavica, 18 (supplement 63): 1, 

 1949) and McKibbin and Taylor ( Journal of 

 Biological Chemistry, 178 : 17, 1949). It 

 was found that this method extracted more 

 strandin and proteolipids than the other 

 methods, but that the extraction of other 

 lipids and removal of non -lipid contami- 

 nants from the extracts were essentially 

 the same for all three methods. 



Sperry (Journal of Biological Chemistry , 

 209 : 377, 1954) found no significant differ- 

 ence in his own method and Folch' s, but 

 his final material was completely soluble 

 in chloroform -methanol (2:1) while Folch's 

 method left a small insoluble residue . 



Folch, J., M. Lees, and G. H. Sloane -Stanley 

 1957. A simple method for the isolation 



_, and purification of total lipids from 



2 animal tissues. Journal of Biological 



• Chemistry , 226 : 497-509. 



A simplification of the procedure of 

 Folch, et al ( Journal of Biological Chemis- 

 try, 19lT833, 1951). 



Tissue lipids are extracted with CHCI3- 

 MeOH (2: 1) and the extract is purified by 

 washing with aqueous salt solutions. 



Forbes, J. C. and T. T. Atkinson, Jr. 



1943. The separate determination of the 

 fatty acid fraction and of the neutral 

 9 fat plus sterol fraction in feces. Jour- 



nal of Laboratory and Clinical Medicine, 

 28: 1507-1510. 

 The fat is extracted and iS separated into 

 free fatty acid and neutral fat fractions by 

 precipitation of the free fatty acids as their 

 Na soaps. The separate portions are then 

 determined by oxidation with silver chromate 

 and iodometric measurement of excess di- 

 chromate . 



Folch, J., I. Ascoli, M. Lees, J. A. Meath, 

 and F.N. LeBaron 



1951. Preparation of lipide extracts from 

 , brain tissue. Journal of Biological 



* Chemistry , 191 : 833-841. 



Lipid Extracts are prepared by homogen- 

 izing brain tissue with CHCl3-MeOH (2:1), 

 filtering to remove insoluble matter, and 



Foreman, H. D. and J. B. Brown 



1944. Solubilities of the fatty acids in 

 organic solvents at low temperatures. 

 • Oil and Soap , 21: 183-187. Chemical 



(1944). 



Abstracts, 38:4821 



Data is given on the solubility in several 

 organic solvents of various saturated and 

 unsaturated fatty acids at temperatures 



22 



