separated on a dichlorodimethylsilane- 

 treated column of silicic acid. Results 

 compared well with ester fractionation 

 methods . 



Garvin, J. E. and M. L. Karnovsky 



1955. Nonaqueous titration of lipids, 

 with particular reference to phospha- 



^ tides and related compounds . Proceed- 



ings of the International Conference on 

 Biochemical Problems of Lipids, 2nd 

 Ghent , (Pub. 1956) pp. 14-16. 

 The use of 99% ethoxyethanol as a solvent 

 for titration of small amounts of fatty acids 

 is discussed. 



Gertler, M. M., J. Kream, and O. Baturay 



1954. Studies on the phosphatide content 

 of human serum. Journal of Biological 

 ^ Chemistry, 207 : 165-173. 



Phosphatides were extracted from serum 

 and hydrolyzed with 6 N HCl for 48 hours 

 at 100°, and the free bases were separated 

 by paper chromatography. Serine and 

 ethanolamine were located on the chroma - 

 togram with ninhydrin and extracted with 

 pyridine. The optical density of the pyri- 

 dine solutions was determined spectrophoto- 

 metrically at 580 mf». Choline was treated 

 on the paper with phosphomolybdic acid- 

 stannous chloride and the spot areas were 

 measured by planimetry. 



Gibble, W. P., E. B. Kurtz, Jr., and 

 A. E. Kelley 



1956. A semi-micro procedure for the 

 separation and degradation of long- 



"^ chain fatty acids . Journal of the Amer - 



# ican Oil Chemists Society, 33: 66-68. 



Chemical Abstracts, 50:5309b (1956). 

 Total lipid is extracted with petroleum 

 ether and saponified by refluxing for 6 hours 

 with 12% alcoholic KOH. The fatty acids are 

 freed with HCl and extracted with ether . 

 The ether is evaporated and the fatty acids 

 are dissolved in acetone and separated by 

 low temperature crystallization. Unsat- 

 urated fatty acids are hydrogenated using 

 Pt02 in absolute ethanol. Saturated fatty 

 acids are degraded using thionyl chloride, 

 pyridine, and AICI3 . 



Click, D. 



1944. Concerning the reineckate method 

 for the determination of choline . Jour - 

 nal of Biological Chemistry, 156 : 643- 

 651. 

 No significant difference in result was ob- 

 tained by the purification of neutralized 

 Ba(0H)2 hydrolysates of wheat germ ex- 

 tracts by ether extraction or by adsorption 

 of the choUne on Permutit and elution with 

 salt solution according to Horowitz and 

 Beadle (Journal of Biological Chemistry , 150 : 

 325, 1943). Choline reineckate was washed 

 free of excess Reinecke salt with n-propanol. 



Goddu, R. F., N. F. LeBlanc, andC. N. Wright 

 1955. Spectrophotometric determination 

 of esters and anhydrides by hydroxamic 



# acid reaction . Analytical Chemistry , 

 27: 1251-1255. 



A method is described for determination 

 of esters by conversion to their hydroxamic 

 acids and spectrophotometric measurement 

 of the color produced by the ferric complexes 

 of the acids . The effects of variables are 

 discussed. 



Goodwin, J. F. 



1959. Total, phospholipide, and labile 

 phosphorus in serum and tissue em- 

 ploying chloric acid and n -phenyl -p- 

 A phenylenediamine . Proceedings of the 



Society for Experimental Biology and 

 Medicine , 100 : 217-219. 

 A colorimetric method is described for 

 determination of the phosphorus fractions 

 of serum. Chloric acid is used for diges- 

 tion, and the phosphomolybdate complex is 

 reduced with p-semidine . The method has 

 the advantages of ease of digestion and 

 color development, and stability of the color 

 complex. It compares well with the Fiske- 

 Subbarow method. 



Gorbach, G. and A. Jurinka 



1944. Micromethods for fats. DC. High- 

 er saturated fatty acids by the Bertram 



• method. Fette und Seifen, 5]_:171-173. 

 Chemical Abstracts, 42:9204f (1948). 



A description of micromodifications of 

 Bertram's method (Zeitschrift fur Unter- 



suchung der Lebensmittel, 55: 179, 1928). 



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