Gortoer, W. A. 



1945. An evaluation of micromethods 

 for phospholipid. Journal of Biological 

 ^ Chemistry , 159 : 97-100. 



Analyses of tissue by determination of 

 lipid phosphorus, by oxidation of the fatty 

 acids from acetone -insoluble lipids, and by 

 direct oxidation of the intact phospholipid 

 are compared. 



When a phospholipid to phosphorus ratio 

 of 24 is used, and a fatty acid recovery of 

 63% of the weight of the saponified phospho- 

 lipid was assumed, the three methods gave 

 comparable results. Phosphorus analysis, 

 however, gave better agreement among 

 replicate samples (error + 0.8%) than either 

 of the oxidative procedures studied (error 

 + 2.2% and + 2.1%). 



The author cautions against the use of 

 conversion factors on mixtures of different 

 types of phospholipid. 



Gracian y Tous, J. and A. V. Pizarro 



1947. Separation of higher fat acids by 

 selective adsorption . Ana les de fisica 



* y quimica (Madrid), 42: 109-122. 



~ Chemical Abstracts, 41:53231 (1947). 



Alumina reacted chemically with oleic and 

 stearic acids, making their separation on 

 alumina difficult. Silicic acid was satis- 

 factory as an adsorbent for the acids . 



Graff, M . M . and E . L . Skau 



1943. Colored chromatograms with hig^- 

 er fatty acids . Industrial and Englneer - 

 J ing Chemistry, Analytical Edition, 15: 



• 340-341 



Fatty acids were separated into zones by 

 adsorption on magnesium oxide impregnated 

 with phenol red. The column was sectioned 

 after development and the fatty acids were 

 recovered by dissolving the MgO in acid and 

 extracting the fatty acids with ether . Sat- 

 urated and unsaturated fatty acids of the 

 same chain length and saturated fatty acids 

 differing in chain length by 4 carbons were 

 separable by the method. 



Griswold, B. L., F. L. Humoller, and 

 A. R. Mclntyre 



1951 . Inorganic phosphates and phos- 

 phate esters in tissue extracts. Ana- 



lytical Chemistry, 23: 192-194. 

 A modification of the Fiske-Subbarow 

 ( Journal of Biological Chemistry , 66: 375, 

 1925) method for reduction of phospho- 

 molybdic acid with aminonaphtholsulfonic 

 acid which uses the heating in 1 N H2SO4 

 recommended by Boltz and Mellon (Ana- 

 lytical Chemistry, 19: 873, 1947) for color 

 development. The higher acidity prevents 

 color formation from reduction of free mo- 

 lybdic acid as is possible in the Fiske- 

 Subbarow method. 



Gurin, S. and D. B. Hood 



1939. The identification and estimation 

 of hexoses in polysaccharides and gly- 

 coproteins by the carbazole method. 

 Journal of Biological Chemistry , 131 : 

 211-223. 

 The. carbazole reaction is used for quanti- 

 tative identification of glucose, fructose, 

 galactose, and mannose, or mixtures of these 

 hexoses, by colorimetric measurement of 

 the colors produced in sulfuric acid. 

 Galactose and an equimolar glucose -mannose 

 mixture give nearly identical values, so a 

 supplementary test is necessary in these 

 cases . 



Hack, M. H. 



1946. Some observations concerning 

 sphingomyelin and sphingomyelin 



▲ reineckate . Journal of Biological Chem - 



istry, 166: 455-462. 



Reineckates prepared from crude extracts 

 contained both glycerol and hexose, implying 

 the presence of other lipids than sphingo- 

 myelin. The MeOH- soluble fraction of ceph- 

 alln and the cerebrosides kerasin and phreno- 

 sin formed reineckates. Recovery of pure 

 sphingomyelins as the reineckates was low. 



Values obtained by isolation of the sphingo- 

 myelin were too low; and those obtained by 

 the Reineckate method were too high, but 

 there was general agreement in thg two meth- 

 ods. 



Hack, M. H. 



1947. Estimation of the phospholipides 

 in human blood. Journal of Biological 

 Chemistry , 169 : 137-143. 



Lipids are extracted from 15 ml. of blood 



25 



