An almost complete reversal in the 

 elution pattern and overlapping of the 

 eluted components occurred when the 

 Hyflo Super-Cel filter aid was heated 

 to 110° before use. 



Hirsh (Journal of Biological Chemistry, 

 233 : 213, 1958) also found that abrupt 

 change of solvent yielded better separa- 

 tions than gradient elution. 



Handler, P. 



1947. The determination of choline in 

 biological material. Biological Sympo - 

 sia, 12: 361-372. Chemical Abstracts, 

 41_:5lT7g(1947). 

 Choline -containing lipid is extracted from 

 tissue with methanol and hydrolyzed with 

 Ba(OH)2 • The choline is precipitated as the 

 reineckate and the precipitate is dissolved 

 in acetone and measured colorimetrically. 



Handschumaker, E. and L. Linteris 



1947. A modified method for the deter- 

 mination of monoglyceride in fats and 

 oils by oxidation with periodic acid. 

 # Journal of the American Oil Chemists 



Society , 24: 143-145. Chemical Ab- 

 stracts , 4]^:4659e (1947). 

 Monoglycerides are determined by peri- 

 odic acid oxidation and iodometric measure- 

 ment of excess periodic acid. 



Hanel, H . K . and H . Dam 



1955. Determination of small amounts of 

 total cholesterol by the Tschugaeff 

 reaction with a note on the determina- 

 tion of latho sterol. Acta chemica 

 Scandinavia , 9: 677-682. 

 A method is described for simultaneous 

 determination of cholesterol and lathosterol 

 (A -7-cholesterol). After hydrolysis with 

 KOH and extraction with petroleum ether, 

 the cholesterol in chloroform solution is 

 treated with ZnCl2 in acetyl chloride and 

 measured in a spectrophotometer at 528myLi. 

 Lathosterol is read at 395 m/x., and gives 

 almost no absorbance at 528 mu. 



Hansen, P. W. and H. Dam 



1957. Paper chromatography and colori- 

 "^ metric determination of free and ester - 



■ ified cholesterol in very small amounts 



of blood. Acta chemica scandinavia, 11: 

 1658-1662. ~ 



Cholesterol is separated by paper chro- 

 matography from substances which might 

 interfere with color development by the 

 method of Zlatkis, Zak, and Boyle (Journal 

 of Laboratory and Clinical Medicine, 41: 

 486, 1958). The method can determine as 

 little as 2.5 fig. of cholesterol and its 

 esters with accuracy of +5%. 



Harding, V.J. and C. E. Downs 



1933. Notes on a Schaffer-Somogyi 



copper reagent. Journal of Biological 



Chemistry, 101 : 487-492- 

 A modified Somogyi copper reagent (Jour- 

 nal of Biological Chemistry, 70: 599, 1926) 

 is recommended as a good general sugar 

 reagent. The reagent is prepared in two 

 parts, and keeps for at least 3 months in 

 the cold. Ammonium salts which interfere 

 in the method are removed with HgSO^ and 

 BaC03 . 



Harris, W. D. and P. Popat 



1954. Determination of the phosphorus 

 content of lipids . Journal of the Amer- 

 A ican Oil Chemists Society, ^: 124-127. 



Chemical Abstracts, 48:6716g (1954). 

 A method is described for determination 

 of lipid phosphorus using perchloric acid 

 digestion and elon(p-methylaminophenolsul- 

 fate) as reducing agent for development of 

 molybdate color . Effects of variables and 

 disadvantages inherent in other methods are 

 discussed. 



Hartman, L. 



1953. Rapid determination of glycerol by 

 the potassium periodate method. Jour- 

 nal of Applied Chemistry, (London) 3: 

 308-311. Chemical Abstracts, 48: 791 

 (1954). 



Complete oxidation of glycerol by KIO4 

 takes place in 3 minutes when an excess of 

 solid KIO4 is used. 



Hartman, L. 



1954. Determination of fat peroxides in 

 the presence of phospholipides. Jour- 



• nal of the Science of Food and Agricul- 



ture, 5: 476-481. Chemical Abstracts, 



27 



