Chemistry , 24: 227, 1916), Myers and 

 Wardell ( Journal of Biological Chemistry, 

 36: 147, 1918), and Reinhold and Shiels 

 (American Journal of Clinical Pathology, 

 6: 22, 1936) were studied. Results from 

 the Bloor and Reinhold and Shiels methods 

 agreed more closely than the Bloor and 

 Myers and Wardell methods . 



Herb, S . F . and R . W . Riemenschneider 



1952. Influence of alkali concentration 

 and other factors on the conjugation of 

 natural polyunsaturated acids as deter - 

 ^ mined by ultraviolet absorption measure- 



ments. Journal of the American Oil 

 Chemists Society, 29: 456-461. Chem - 

 ical Abstracts, 47:888e (1953). 

 Optimum conditions for maximum conjuga- 

 tion of methyl arachidonate were found to be 

 15 minutes heating at 180°C in 21% KOH- 

 glycerol. Other acids treated accordingly 

 provided greater sensitivity in the spectro- 

 photometric method. 



Journal of Laboratory and Clinical 



Medicine , 32: 1163-1168. 

 Cholesterol esters and cholesterol are 

 separated by adsorption on alumina and 

 elution with 10% ethyl ether in petroleum 

 ether and 10% ethanol in petroleum ether, 

 respectively. The esters are saponified, 

 and cholesterol is determined in both frac- 

 tions by the Liebermann-Burchard method. 



Hill, U. T. 



1946. Colorimetric determination of 

 fatty acids and esters. Industrial and 

 Engineering Chemistry, Analytical 



Edition, 18: 317-319. 

 A method is described for determination 

 of fatty acids and esters by conversion to 

 their hydroxamic acid derivatives and col- 

 orimetric measurement of the color pro- 

 duced with Fe"*"*"*". The fatty acids are 

 methylated before treatment. Accuracy of 

 the determinations was within + 0.01 mg. 

 of cottonseed oil. 



Herb, S . F . and R . W . Riemenschneider 



1953. Spectrophotometric micromethod 

 for determining polyunsaturated fatty 

 9 acids. Analytical Chemistry, 25: 953- 



955. 

 A method is described for determination 

 of fatty acids with 2-5 double bonds in a 

 1-10 mg. sample of fat. The acids are 

 isomerized, and the density of a methanolic 

 solution of the isomerized acids is measured 

 spectrophotometrically. Results by the 

 method agree with macromethods. 



Herbain, M. 



1959. Estimation of blood cholesterol 

 by colorimetry, with the use of blood 

 coagulant. Process improvements, 

 particularly in the case of high hyper- 

 lipemias . Bulletin de la Societe de 

 chimle biologique, 41: 821.-833 . Chem- 

 ical Abstracts, 53:22188e (1959). 

 Several modifications of the Sperry and 



Webb method for cholesterol determination 



are proposed. 



Hess, W. C. 



"^ 1947. Chromatographic separation of cho- 



" lesterol and cholesterol esters in blood. 



Hill, U. T. 



1947. Colorimetric determination of 

 ^ fatty acids and esters. Analytical 



Chemistry , J^: 932-933. 

 A modification of Hill's method (Indus - 

 trial and Engineering Chemistry, Analytical 

 Edition , 18: 317, 1946) which gives a more 

 stable color. 



Hirsch, J. and E. H. Ahrens, Jr. 



1958. The separation of complex lipide 

 mixtures by the use of silicic acid chro- 

 -^ matography . Journal of Biological Chem- 



istry, 233: 311-320. 

 Complex lipid mixtures are separated into 

 chemical classes by elution from a single 

 silicic acid column. Synthetic mixtures and 

 plasma lipids were used to test the method. 



Hodgson -Jones, I. S. and V. R. Wheatley 



1952. Studies of sebum. 3. Methods for 

 the collection and estimation of small 

 amounts of sebum . Biochemical Jour- 

 nal, 52: 460-464. 

 Various methods of collection and estima- 

 tion of sebum are discussed. Carbon tetra- 

 chloride was found to be more suitable than 

 ether, ethanol, or acetone for removal of 



29 



