sebum from t±e skin. Gravimetry, nephelo- 

 metry, and chromate oxidation were found 

 to be suitable methods for estimation of the 

 sebum collected. 



Holasek, A. andK. Winsauer 



1954. Separation of saturated fatty acids 

 by paper chromatography. Monatshefte , 



* 85:796-801. Chemical Abstracts, 49: 



• 2757a (1955). 



C4-C18 saturated fatty acids were sepa- 

 rated on a potassium alum -impregnated 

 filter paper using CCl4-MeOH-conc. 

 NH4OH (81:18:1) as developer. They were 

 located by spraying with Rhodamine B in 

 HCl and viewing under LTV light . 



Holman, R. T. andG. O. Burr 



1948. Alkali conjugation of the unsatu- 

 ^ rated fatty acids . Archives of Biochem - 



• istry , 29: 474-482. 



A study of the effects of KOH concentra - 

 tion and time of reaction on conjugation of 

 polyunsaturated fatty acids . 



Holman, R . T . and L . Hagdahl 



1950. Displacement analysis of lipides. 



III. Separation of normal saturated 

 ■^ fatty acids from formic to behenic . 



# Journal of Biological Chemistry, 182 : 

 421-427. 



The fatty acids were adsorbed on a Darco 

 G-60 column and separated by displacement 

 analysis using water, alcohol-water mix- 

 tures, and chloroform -alcohol as solvents. 

 Yields were not studied. 



Holman, R. T. 



1951. Displacement analysis of lipids. 



IV. Carrier displacement separation 

 -^ of saturated fatty acids . Journal of the 



# American Chemical Society, 73 : 1261- 

 1263. 



The saturated fatty acids are separated 

 by carrier displacement on charcoal using 

 their methyl esters as carriers. 5-15 mg. 

 quantities of lauric, myristic, palmitic, 

 and stearic acids are nearly 100% recover- 

 able. 



Holman, R. T. 



"At 1951. Displacement analysis of lipids . 



V. Separation cf substances analogous 

 to fatty acids . Journal of the American 

 Chemical Society, 73: 3337-3340. 

 Various aliphatic compounds were stud- 

 ied for use as carriers for displacement 

 separations. A series of C,g compounds 

 of varying adsorbability which are suitable 

 for use as displacers is given. 



Holman, R. T. and W. T. Williams 



1951. Displacement analysis of lipids. 

 i^ VI. Separation of unsaturated acids. 



^ Journal of the American Chemical So- 



• ciety, 73: 5285-5289. 

 Unsaturated fatty acids were separated 



by displacement chromatography on char- 

 coal . Saturated and unsaturated acids of 

 4-18 C with the same chain length were sep- 

 arated. Effects of conjugation on adsorption 

 characteristics are discussed. 



Holman, R. T. 



1951. Displacement analysis of lipids. 

 4_ VII. Carrier separation of unsaturated 



^ fatty acids . Journal of the American 



• Chemical Society , 73:5289-5292. 



A method for separation of unsaturated 

 fatty acids on charcoal using methyl esters 

 as carriers is described. Effects of unsatu- 

 ratlon on adsorbability are discussed. 



Holman, R. T., H. Hayes, and 

 P. R. Edmondson 



1957. Analysis of unsaturated fatty acids. 

 Essential Fatty Acids. Proceedings of 

 the International Conference on Biochem - 



• ical Problems of Lipids, 4th Oxford, 

 (Pub. 1958) pp. 9-15. Chemical Abstracts, 

 53:17277f (1959). 



Methods are given for analysis of unsatu- 

 rated fatty acids by alkali isomerization (Hol- 

 man, in Methods of Biochemical Analysis, 

 (D. Click, Ed.) 4: 99, 1957) paper chroma - 

 tography (Mangold, et al. Journal of the Amer- 

 ican Chemical Society, ~77: 6070, 1956), and 

 near-infra-red spectrophotometry (Holman 

 and Edmondson, Analytical Chemistry, 28: 

 1533, 1956). 



Horecker, B. L., T. S. Ma, andE. Haas 



1940. Note on the determination of micro 

 quantities of organic phosphorus. 



30 



