Journal of Biological Chemistry , 136 : 



775-776. 

 A modified Fiske-Subbarow method (Jour- 

 nal of Biological Chemistry , 66 : 375, 1925) 

 which will determine Ijdg. of phosphorus 

 with an accuracy of + 3% is described. A 

 sulfuric acid concentration of 2 N is used 

 in place of the original 0.5 N, and the color 

 Is developed with heat. 



Hornstein, I., J. A. Alford, L. E. Elliot, and 

 P. F. Crowe 



1960. Determination of free fatty acids 



-^ in fat. Analytical Chemistry , 32 : 540- 



• 542. 



Free fatty acids are separated from fat 

 mixtures by adsorption on Amberlite IRA- 

 400 resin. Fat is removed by washing the 

 resin with petroleum ether . The fatty acids 

 are converted to their methyl esters by 

 treatment, while on the resin, with metha- 

 nolic HCl and are separated and identified 

 by gas chromatography on a polyvinyl ace- 

 tate column. 



Horowitz, N. H. and G. W. Beadle 



1943. A microbiological method for the 

 determination of choline by use of a 

 mutant of neurospora. Journal of Bio - 

 logical Chemistry , 150: 325-333. 

 A simple, sensitive (0.02 mg. choline/ 

 liter), and specific method. Interfering sub- 

 stances are removed from the solution by 

 adsorption on Permutlt, and choline is 

 eluted with 5% NaCl. Choline is determined 

 by microbiological assay using a choline - 

 less strain of Neurospora crassa . 



Howard, G. A. and A. J. P. Martin 



1950. The separation of the C12-C18 

 . fatty acids by reversed phase partition 



^ chromatography. Biochemical Journal, 



• 46: 532-538, 



A method is given for the separation of 

 the fatty acids on a dichlorodimethylsilane- 

 impregnated silicic acid column. 



Huennekens, F. M., D. J. Hanahan, and 

 M. Uziel 

 ^^ 1954. Paper chromatography of lecithins. 



Journal of Biological Chemistry, 206: 

 ^ 443-447. 



A series of compounds derived from 

 (dipalmitoleyl)-L- a-glycerylphosphoryl- 

 choline are separated by paper chromatog- 

 raphy, using alcohol -water mixtures as 

 solvent systems. 



Hunter, M. O., R. A. Knouff, and 

 J. B. Brown 



1945. The microdetermination of tissue 

 lipids . Ohio Journal of Science, 45: 



• 47-54. Chemical Abstracts, 39: 3804^ 

 (1945). 



Methods are described for the extraction 

 and saponification of tissue lipids and titri- 

 metric estimation of 1-10 mg. of fatty acids 

 to within 2%. Other methods are also dis- 

 cussed. 



It was found that liver and adrenal tissue 

 lipids were completely extracted by a 5 hour 

 extraction with 3:1 alcohol -ether at room 

 temperature. The precipitation of choles- 

 terol as the digitonide by the Schoenheimer- 

 Sperry method was found to be incomplete 

 for samples of 0. 1 to 0.5 mg. 



Dceda, R. M., A. D. Webb, andR. E. Kepner 

 1954. Chromatographic separation of 

 j^ p-phenylazophenacyl esters on silicic 



^ acid. Analytical Chemistry, 26: 1228- 



• 1229. 



A method is described for the separation 

 of fatty acids by chromatography as their 

 p-phenylazophenacyl esters on silicic acid 

 using benzene and Skellysolve B mixtures as 

 developing solvents. 



Inouye, Y. and J. Yukawa 



1940. Separation and identification of 

 aliphatic acids. I. Hydroxamic acids 

 , derived from saturated aliphatic acids. 



"*^ Journal of the Agricultural Chemical 



• Society of Japan, 16: 504-509. Chemical 

 Abstracts, 35^:730"9(1941). 



Hydroxamic acid derivatives were prepared 

 from ethyl esters of fatty acids by reaction 

 with hydroxylamine hydrochloride and sodium 

 ethylate at room temperature . Data on the 

 melting points of the derivatives are given. 



Inouye, Y . and M . Noda 

 ■A" 1950. Separation and identification of 



• fatty acids. IX. Paper partition chro- 



31 



