26: 634, 1952) for saturated fatty acids was 

 applied to unsaturated fatty acids. R^ val- 

 ues for 14 unsaturated fatty acids are given. 



Inouye, Y . and M . Noda 



1955. Separation and identification of 

 fatty acids. XVII. Paper chromatog- 

 raphy of saturated fatty acids as their 

 •if 2, 4-dinitrophenylhydrazides. Bullet in 



of the Agricultural Chemical Society of 



Japan, j^: 214-219 (In English). Chem - 

 ical Abstracts, 50: 17478b (1956). 

 2, 4-dinitrophenylhydrazides were obtained 

 by reaction of fatty acid chlorides with 10%, 

 solution of 2,4-dinitrophenylhydrazine in 

 pyridine. The hydrazides were chromato- 

 graphed on tetralin -impregnated paper using 

 tetralin-AcOH-EtOH or MeOH mixtures as 

 mobile phase . The spots were located by 

 spraying with 0.5 N KOH in EtOH. Data on 

 melting points of the hydrazine derivatives 

 and their R^ values with the various solvents 

 are given. 



Inouye, Y., M. Noda, and O. Hirayama 



1955. Paper chromatography of unsatu- 

 rated fatty acid esters as their mercuric 



■ acetate addition compounds . Journal of 



the American Oil Chemists Society, 3J: 



• 132-135. Chemical Abstracts, 49:6783f 

 (1955). 



Methyl esters of unsaturated fatty acids 

 were chromatographed as their mercuric 

 acetate addition compounds and were detec- 

 ted by treatment with diphenylcarbazone . 

 Rj values of 19 of the addition compounds 

 are given. 



Inouye, Y., O. Hirayama, andM. Noda 



1956. Separation and identification of 

 fatty acids. XVni. Paper chromatog- 



. raphy of fatty acids as their acetol 



^ ester derivatives. Journal of Japan Oil 



* Chemists' Society , _5: 16-18. Chem- 

 ical Abstracts, 50:17478e (1956). 



Acetol esters were obtained by reaction 

 of K soaps of the fatty acids with 

 BrCH2COCH3 and converted to z, 4-dinitro- 

 phenylhydrazones and thiosemicarbazones. 

 The derivatives were chromatographed on 

 Decalin -impregnated paper with mixtures of 

 MeOH, Decalin, AcOH, and EtOH as sol- 



vents. Melting points of acetol esters and 

 Rj values of the derivatives are given. 



Inouye, Y., O. Hirayama, andM. Noda 



1956. Separation and identification of 

 fatty acids. XX. 2, 4-dinitrophenyl- 

 hydrazones of p-bromophenacyl esters 

 as derivatives for characterization of 



# unsaturated fatty acids . Bulletin of the 

 Agricultural Chemical Society of Japan, 

 20: 194-199. Chemical Abstracts, Sh 

 7297f (1957). 



A method is described for the preparation 

 of the p-bromophenacyl ester-2, 4-dinitro- 

 phenylhydrazone derivatives of the C2-C20 

 fatty acids with even -numbered carbon chains 

 for use as identifying derivatives for the un- 

 saturated fatty acids . 



Inouye, Y., O. Hirayama, andM. Noda 



1956. Separation and identification of 

 fatty acids. XXI. Paper chromatog- 

 raphy of fatty acids as their p-bromo- 



■^ phenacyl ester derivatives. Bulletin of 



# the Agricultural Chemical Society of 

 Japan, 20:200-205. Chemical Abstracts, 

 51:7297g(1957). 



A method is described for the separation 

 of fatty acids by paper chromatography as 

 their p-bromophenacyl ester-2, 4-dinitro- 

 phenylhydrazone derivatives and their mer- 

 curic acetate addition compounds . 



Inouye, Y., O. Hirayama, andM. Noda 



1957. Separation and identification of 

 fatty acids. XXII. Quantitative analysis 



j^ of fatty acids by paper chromatography. 



^ Journal of the Agricultural Chemical 



# Society of Japan, 31_: 568-572. Chemical 

 Abstracts, 53: 2084 If (1959). 



The p-bromophenacyl ester-2, 4-diiutro- 

 phenylhydrazones and Hg(0Ac)2 derivatives 

 of the fatty acids were separated by paper 

 chromatography, extracted with benzene, 

 and measured spectrophotometrically. 



Ireland, J.T. 



1941. The colorimetric estimation of 



total cholesterol in whole blood, serum, 

 ■ plasma, and other biological material . 



Biochemical Journal, 35: 283-293. 

 The effects of ligjit, reagent concentra- 



33 



