Chemistry, 25: 813, 1953). 



and used as eluent. 



Jones, K. K. 



1950. A micromethod for fat analysis 

 based on formation of monolayer films. 

 ^ Quarterly Bulletin of Northwestern 



* University Medical School , 24:253-256. 



Chemical Abstracts, 45:5754a (1951). 

 A method is described for estimation of 

 lipids by spreading them in monomolecular 

 films on an acidified water solution and 

 measuring the area of the films. 



Kaufmann, H. P. and W. Wolf 



1943. Adsorption separation in the field 

 of fats. V. The separation of cis-trans 

 ic isomers. Fette und Seifen, 50: 519- 



• 521. Chemical Abstracts, 39r205^ 



(1945). 

 A comparative study and discussion of the 

 use of AI2O3, Si02, and charcoal as adsorb- 

 ents for the separation of isomeric fatty 

 acids. 



Jorgensen, K. and H. Dam 



1957. An ultramicro method for the 

 determination of total cholesterol in 

 bile based on the Tschugaeff color re- 

 action . Acta chemica Scandinavia, 1 1 : 

 1201-120"8^ 

 A method is described for measurement 

 of as little as 2^g. of cholesterol in a final 

 volume of reaction mixture of 0.5 ml. Hy- 

 drolysis, extraction and color development 

 are all carried out in the same tube. The 

 developed color is read spectrophotometric- 

 ally. 



Kabara, J. J. 



1954. The light insensitivity of the 



Liebermann-Burchard reaction during 

 spectrophotometric determination of 

 cholesterol. Journal of Laboratory and 

 Clinical Medicine, 44: 246-249. 

 It was found that exclusion of light during 

 development of color with the Liebermann- 

 Burchard reaction was unnecessary unless 

 light of 340-540 m/. was used for measure- 

 ment. Light had no effect on the L-B reac- 

 tion from 580-740 m u . in either polar or 

 nonpolar solvents. 



Kapitel, W. 



1956. Quantitative column chromatograph- 

 A ic separation of mixtures of fatty acids . 



^ Fette, Seifen, Anstrichmittel , 58: 91-94. 



• Chemical Abstracts, 50:9761c (1956). 



The C5-C23 saturated fatty acids were 

 separated by chromatography on a column of 

 kieselguhr which was impregnated with par- 

 affin and treated with dichlorodimethyl si- 

 lane. A mixture of various amounts of water 

 and acetone was saturated with paraffin oil 



Kaufmann, H. P. and J. Budwig 



1950. The foam test in paper chromatog- 

 _ raphy. Fette und Seifen, 52: 555-556. 



Chemical Abstracts, 45:2236d (1951) . 

 As little as 10// g. of oleic acid is detect- 

 able by using an adaptation of the foam test 

 for chromatography. The fatty acid or soap 

 is placed on a copper acetate-impregnated 

 paper and a H2O2-NH4OH reagent is added, 

 causing it to foam . 



Kaufmann, H. P. 



1950 . New methods of fat analysis . 

 Fette und Seifen , 52: 713-721. Chem- 



• ical Abstracts, 45:8271a (1951). 

 Various methods suitable for identification 



of traces of fats and fatty acids on paper chro- 

 matogramsare described. 



Kaufmann, H . P . and J . Budwig 



1951. Paper chromatography in the fat 

 field, rv. Radiometry of oleic acid. 



• Fette und Seifen, 53: 69-73. Chemical 

 Abstracts, 45:9893e (1951). 



Fatty acid is determined by formation of 

 Co"^ soaps and radiation counting. 



Kaufmann, H. P. and J. Budwig 



1951. Paper chromatography in the fat 

 field. V. Radiometric determination of 

 the iodine number. Fette und Seifen, 53: 



• 253-259. Chemical Abstracts, 45:78017 

 (1951). 



An adaptation of the Hanus method for io- 

 dine number determination for use in paper 

 chromatography. The iodine number is de- 

 termined by measurement of the radiation 

 from the I^-^^ added by the fat. 



35 



