change in absorption caused by enzymic 

 oxidation of triphosphopyridine nucleotide 

 in the presence of glucuronate . 



Kennedy, E. P. andH. A. Barker 



1951. Paper chromatography of volatile 

 ■^ acids. Analytical Chemistry , 23 : 1033- 

 • 1034. 



A method is described for chromatography 

 on paper of ammonium salts of the volatile 

 fatty acids using solvents containing free am- 

 monia for development. Bromophenol blue 

 is used to locate the spots after development. 



Kenny, A. P. 



1952. The determination of cholesterol 

 by the Liebermann-Burchard reaction. 

 Biochemical Journal, 52: 611-619. 



A modification of the L-B method for es- 

 timation of total cholesterol in serum or 

 plasma. The yellow component is measured 

 at 430 mfi. The method is suitable for rou- 

 tine clinical work. The factors influencing 

 color development in the determination of 

 cholesterol by the L-B reaction were studied 

 and are discussed. 



Kepner, R. E., A. D. Webb, R. L. King, and 

 A. D. Bond 



1957. p-Phenylazophenacyl esters. 



Rates of movement relative to p-phenyl- 

 j azophenacyl bromide on silicic acid and 



^ identification by paper partition chroma - 



tography. Analytical Chemistry , 29 : 

 1162-1164. 

 An improved method for the preparation 

 of p-phenylazophenacyl bromides and a meth- 

 od for paper chromatography of p-phenylazo- 

 phenacyl esters are described. Rates of 

 travel of the p-phenylazophenacyl derivatives 

 on siUcic acid as compared to phenylazo- 

 phenacyl bromide are given. Melting points 

 of 30 derivatives are listed. 



Kerr, L. M. H. and W. S. Bauld 



1953. The chromatographic separation of 

 "A" free and combined plasma cholesterol. 

 ■ Biochemical Journal, 55 : 872-875. 



Acetone -ethanol extracts of plasma were 

 chromatographed on alumina using petrole- 

 um ether and benzene as eluting solvents for 

 the separation of cholesterol and cholesterol 



esters. Both the cholesterol and the esters 

 were estimated by the Liebermann-Burchard 

 color reaction, using separate standard 

 curves for the cholesterol and the esters. 

 Accuracy of the method is lower than the 

 Schoenheimer-Sperry method. 



Ketchum, D. 



1946. Semimicrodetermination of sapon- 

 ification equivalent by Rieman's double - 

 indicator method. Industrial and Engi- 

 neering Chemistry, Analytical Edition, 

 18: 273-274. 

 A semimicro modification of Rieman's 

 method (I ndustrial and Engineering Chemis - 

 try, Analytical Edition , 15: 325, 1943). 



Kibrick, A. C, T. Roberts, andS. Skupp 

 1951. Determination of cholesterol in 

 blood plasma or serum by hydrolysis 

 with benzyltrimethylammonium hydrox- 

 ide. Archives of Biochemistry and Bio- 

 physics, 32: 9-13. 

 The cholesterol esters are hydrolyzed with 

 benzyltrimethylammonium hydroxide during 

 evaporation of the alcohol-ether tissue ex- 

 tract. The color is developed in the residue 

 by the Liebermann-Burchard reaction and 

 read colorimetrically. Recovery of 89-104% 

 of added cholesterol was obtained. The meth - 

 od compares favorably with the Schoenheimer- 

 Sperry method. 



Kibrick, A. C, L. B. Safier, andS. J. Skupp 

 1959. Existence of fatty acid peroxides 

 in normal blood and tissues of man and 



# animals. Proceedings of the Society 

 for Experimental Biology and Medicine, 

 101 : 137-139. 



The thiobarbituric reaction (Kohn and 

 Liver sedge. Journal of Pharmacology and 

 Experimental Therapeutics, 82: 292, 1944) 

 was modified for determination of fatty acid 

 peroxides in blood as well as in tissues. 



Kibrick, A. C. andS. J. Skupp 



1959. Chromatographic separation of 

 , fatty acids based on chlorophenacyl 



^ esters. Analytical Chemistry , 31: 



* 2057-2060. ~ 



A method is described for synthesis of 

 4'-bromo-2-chloroacetophenone and prep- 



38 



