aration of its fatty acid esters. The fatty 

 acid esters are separated on a polyethylene 

 column using various water-alcohol mix- 

 tures as eluents. Melting points, solubil- 

 ities, and absorptivities of the derivatives 

 are given. 



A drawing of a reservoir system for 

 maintaining constant pressure while changing 

 solvents is included. 



King, E. J. 



1932. The colorimetric determination of 

 phosphorus. Biochemical Journal , 26: 

 ^ 292-297. 



Perchloric acid is used for oxidation of 

 organic matter. l:2:4-aminonaphtholsul- 

 fonic acid is used as a reducing agent to de- 

 velop molybdate color. 



Kingsley, G . R . and R . R . Schaffert 



1949. Determination of free and total 

 cholesterol by direct chloroform ex- 

 A traction. Journal of Biological Chemis- 



try, 180 : 315-328. 

 Cholesterol is extracted from 0.2 ml. of 

 serum with chloroform . The extract is 

 dried with anhydrous magnesium sulfate. 

 Acetic anhydride and sulfuric acid are added 

 to a portion of the dried extract and the col- 

 or is read photometrically. 



Kinley, L. 



1958. Serum cholesterol determinations 

 as affected by vitamin A. Proceedings 

 -A of the Society for Experimental Biology 



and Medicine, 99: 244-245. 

 Very high vitamin A levels will increase 

 the cholesterol levels as measured by the 

 Zak method due to interference with the fer- 

 ric chloride reagent. Moderate levels have 

 little effect. Cholesterol values determined 

 by the Schoenheimer-Sperry method were 

 not affected by vitamin A. 



Kirchner, J. G., A. N. Prater, and 

 A.J. Haagen-Smit 



1946. Separation of acids by chromato- 

 graphic adsorption of their p -phenyl - 

 ■^ phenacyl esters. Industrial and Engi- 



neering Chemistry, Analytical Edition, 



18: 31-32. 

 A method is described for the separation 



of fatty acids by chromatography of their 

 p-phenylphenacyl esters on silicic acid. 

 Good separations were obtained. 



Kirk, E., I. H. Page, and D. D. Van Slyke 



1934. Gasometric microdetermination of 

 # lipids in plasma, blood cells, and tissue. 



A Journal of Biological Chemistry , 106: 



■ 203-234. 



After alcohol-ether (3:1) extraction, ali- 

 quots of the extract are used for determina- 

 tion of lipid constituents . Total lipids are 

 estimated by determination of total non- vol- 

 atile carbon. Cholesterol is determined 

 by combustion of the digitonide. Phospho- 

 lipids are estimated as phosphoric acid by 

 precipitation as strychnine phosphomolyb- 

 date and determination of carbon in the pre- 

 cipitate . 



The maximum temperature of 60° was 

 found to be critical in the evaporation of the 

 alcohol-ether extract, as reextraction with 

 petroleum ether was incomplete if a higher 

 evaporation temperature was used. 



Acetone -MgCU precipitates all but 2 to 

 3% of the phospholipids, but all the precip- 

 itated phospholipid is not soluble in moist 

 ether. 



Kirk, E. 



1938. A study on Kimmelsteil's procedure 

 for titrimetric cerebroside determina- 

 tion, with description of an improved 

 technique. Journal of Biological Chem - 

 istry , ^2^: 613-621. 

 Kimmelsteil's procedure (Biochemische 

 Zeitschrift, 212:259, 1929) was found to 

 give high values for cerebroside due to the 

 presence of interfering reducing substances. 

 A modification is presented which removes 

 these substances by precipitation from the 

 hydrolyzed sample with zinc hydroxide. The 

 method is suitable for determination of 0.3 

 to 1.3 mg. of pure cerebroside (+4%) and 

 gives quantitative recovery of added cerebro- 

 side. 



Kirk, E. 



1938. A micro method for approximate 

 estimation of lecithin, cephalin, ether- 

 ▲ insoluble phosphoinositide, and cerebro- 



sides in plasma, red blood cells, and 



39 



